中华麻醉学杂志
中華痳醉學雜誌
중화마취학잡지
CHINESE JOURNAL OF ANESTHESIOLOGY
2009年
3期
233-235
,共3页
麻醉药,吸入%认知障碍%老年人
痳醉藥,吸入%認知障礙%老年人
마취약,흡입%인지장애%노년인
Anesthetics,inhalation%Cognition disorders%Aged
目的 评价反复吸入七氟烷对老年大鼠认知功能的影响.方法 健康Wistar大鼠24只,月龄18月,体重270-450 g,雌雄各半,随机分为3组(n=8):对照组(C组)、2%七氟烷组(S1组)和3%七氟烷组(S2组).C组不吸入七氟烷;S1和S2组分别吸入2%、3%七氟烷,100 min/d,连续5d.于干预结束后1-6d(T1-6)时进行水迷宫实验,记录T1-5时游泳时间和游泳距离及T6时第4象限探索时间和游泳距离.于最后1次水迷宫实验结束后60 min处死大鼠,取海马组织,采用RT-PCR法测定海马N-甲基肌天冬氨酸受体(NMDAR)、NR1和NR2B mRNA的表达.结果 与C组比较,S1组T1时游泳时间和游泳距离延长.T6时第4象限探索时间缩短,NMDAR mRNA表达上调(P<0.05),NRI和NR2B mRNA的表达差异无统计学意义(P<0.05).结论 反复吸入2%七氟烷不会导致老年大鼠认知功能减退;而反复吸入3%七氟烷可导致一过性认知功能减退.
目的 評價反複吸入七氟烷對老年大鼠認知功能的影響.方法 健康Wistar大鼠24隻,月齡18月,體重270-450 g,雌雄各半,隨機分為3組(n=8):對照組(C組)、2%七氟烷組(S1組)和3%七氟烷組(S2組).C組不吸入七氟烷;S1和S2組分彆吸入2%、3%七氟烷,100 min/d,連續5d.于榦預結束後1-6d(T1-6)時進行水迷宮實驗,記錄T1-5時遊泳時間和遊泳距離及T6時第4象限探索時間和遊泳距離.于最後1次水迷宮實驗結束後60 min處死大鼠,取海馬組織,採用RT-PCR法測定海馬N-甲基肌天鼕氨痠受體(NMDAR)、NR1和NR2B mRNA的錶達.結果 與C組比較,S1組T1時遊泳時間和遊泳距離延長.T6時第4象限探索時間縮短,NMDAR mRNA錶達上調(P<0.05),NRI和NR2B mRNA的錶達差異無統計學意義(P<0.05).結論 反複吸入2%七氟烷不會導緻老年大鼠認知功能減退;而反複吸入3%七氟烷可導緻一過性認知功能減退.
목적 평개반복흡입칠불완대노년대서인지공능적영향.방법 건강Wistar대서24지,월령18월,체중270-450 g,자웅각반,수궤분위3조(n=8):대조조(C조)、2%칠불완조(S1조)화3%칠불완조(S2조).C조불흡입칠불완;S1화S2조분별흡입2%、3%칠불완,100 min/d,련속5d.우간예결속후1-6d(T1-6)시진행수미궁실험,기록T1-5시유영시간화유영거리급T6시제4상한탐색시간화유영거리.우최후1차수미궁실험결속후60 min처사대서,취해마조직,채용RT-PCR법측정해마N-갑기기천동안산수체(NMDAR)、NR1화NR2B mRNA적표체.결과 여C조비교,S1조T1시유영시간화유영거리연장.T6시제4상한탐색시간축단,NMDAR mRNA표체상조(P<0.05),NRI화NR2B mRNA적표체차이무통계학의의(P<0.05).결론 반복흡입2%칠불완불회도치노년대서인지공능감퇴;이반복흡입3%칠불완가도치일과성인지공능감퇴.
Objective To investigate the effects of repeated inhalation of sevoflurane on cognitive function in aged rats. Methods Twenty-four Wistar rats of beth sexes aged 18 months weighing 270-450 g were randomly divided into 3 groups(n=8 each):control group(C),2%aevoflurane group(S1)and 3% sevoflurane group (S2).Group C inhaled oxygen(2.5 L/min)and nitrogen(6L/min)only, while group S1 and S2 inhaled 2%and 3%sevonurane in oxygen(2.5 L/min)and nitrogen(6 L/min)100 min/d for 5 d respecfivelv. The Morris watemaze test was performed once a day for 6 consecutive clays after the last inhalation(T1-6).The swimming time and distance at T1-5 and probe time and swimming distance in the fourth quadrant at T6 were recorded. The aninlala were killed within 60 min after the last test and the hippecampus were immediately removed for determination ofthe expression of NMDAR, NRI and NR2B mRNA using RT-PCR.Results The swimming time and disl .ance were significantly longer at T1,and the probe time in the fourth quadrant shorter and expression of NMDAR mRNA hisher at T6 in group s2 than in group C(P<0.05).Conclusion Repeated inhalation of 2% sevoflurane can not induce cognitive disordel-,while 3%sevoflurane Call induce transient cognitive decline in aged rats.
