CAJ | 학술논문

目的探讨不同浓度氯胺酮、羟丁酸钠对福尔马林致痛小鼠胃肠推进运动的影响。方法实验小鼠按随机数字法分层随机，分为疼痛模型组（32只）、静脉麻醉药组（24只）、静脉麻醉药+疼痛模型组（56只）共3组。各组均采用炭末胶液（含12％活性炭和4％黄芪胶）灌胃，计算30 min炭末胶液在小肠内推进距离占小肠全长的百分比，以此作为胃肠推进运动的指标。小鼠足底皮下分别注射2％、4％、6％浓度福尔马林溶液建立福尔马林炎性疼痛模型。结果①与正常对照组相比，2％福尔马林疼痛模型组小鼠胃肠推进运动明显减慢[(45±9)％vs(54±ll)％，P＜0.05]，而4％及6％福尔马林疼痛模型组小鼠胃肠推进运动差异无统计学意义。②与生理盐水组相比，麻醉剂量氯胺酮和羟丁酸钠组小鼠胃肠推进运动虽有不同程度减慢，但差异无统计学意义（P＞0.05）。③在4％福尔马林疼痛模型下，与生理盐水组相比，麻醉剂量氯胺酮组增加小鼠胃肠推进运动[(69±l1)％vs(61±7)％，P＜0.05]，镇痛剂量[(59±7)％]与小剂量氯胺酮组[(61±6)％]对胃肠推进运动影响甚微(P＞0.05)，麻醉剂量[(48±5)％]与小剂量羟丁酸钠组[(52±6)％]胃肠推进运动减慢(P＜0.05)。结论2％福尔马林疼痛模型组小鼠胃肠推进运动明显减慢，腹腔注射麻醉剂量氯胺酮能促进疼痛刺激后小鼠的胃肠推进运动，而麻醉剂量和小剂量羟丁酸钠则抑制小鼠的胃肠推进。
목적탐토불동농도록알동、간정산납대복이마림치통소서위장추진운동적영향。방법실험소서안수궤수자법분층수궤，분위동통모형조（32지）、정맥마취약조（24지）、정맥마취약+동통모형조（56지）공3조。각조균채용탄말효액（함12％활성탄화4％황기효）관위，계산30 min탄말효액재소장내추진거리점소장전장적백분비，이차작위위장추진운동적지표。소서족저피하분별주사2％、4％、6％농도복이마림용액건립복이마림염성동통모형。결과①여정상대조조상비，2％복이마림동통모형조소서위장추진운동명현감만[(45±9)％vs(54±ll)％，P＜0.05]，이4％급6％복이마림동통모형조소서위장추진운동차이무통계학의의。②여생리염수조상비，마취제량록알동화간정산납조소서위장추진운동수유불동정도감만，단차이무통계학의의（P＞0.05）。③재4％복이마림동통모형하，여생리염수조상비，마취제량록알동조증가소서위장추진운동[(69±l1)％vs(61±7)％，P＜0.05]，진통제량[(59±7)％]여소제량록알동조[(61±6)％]대위장추진운동영향심미(P＞0.05)，마취제량[(48±5)％]여소제량간정산납조[(52±6)％]위장추진운동감만(P＜0.05)。결론2％복이마림동통모형조소서위장추진운동명현감만，복강주사마취제량록알동능촉진동통자격후소서적위장추진운동，이마취제량화소제량간정산납칙억제소서적위장추진。
Objective To investigate the effects of different dose of ketamine and sodium oxybate on gastrointestinal peristalsis in mice model of formalin-induced pain. Methods The mice were divided into three groups by Random number method and Stratified sampling: pain model group (n=32), intravenous anesthetic group (n=24), and pain model+intravenous anesthetic group(n=56). During the experiment, a meal consisting of an aqueous suspension(0.3 ml) of 12％ charcoal, used as a marker, and 4％ tragacanth mucilage was administered intragastrically. The percentage distance traveled by the charcoal plug in the small intestine (from the pylorus to the caecum) was measured as the gastrointestinal transit 30 min after receiving the test meal. The mice received an intra plantar injection of different concentration (2, 4, 6％ ) of formalin solution as formalin induced pain model. Results (① The 2％ dose of formalin significantly reduced the rate of gastrointestinal motility in mice [ (45±9)％ vs (54±11 )％, P＜0.05 ], while the 4％ and 6％ dose of formalin had little influence on gastrointestinal motility than that in black group. ② The intraperitoneal injection of anesthetic-doses of ketamine and sodium oxybate slowed down the gastrointestinal motility in mice, but had no statistical significance than that in normal saline group (P＞0.05). ③ For formalin-induced pain model, compared with normal saline group the anesthetic-dose of ketamine increased the rate of gastrointestinal motility in mice [ (69±11)％ vs (61±7)％, P＜0.05 ]. Analgesic-dose and low-dose of ketamine[(59±7)％ and (61±6)％]had little effect on gastrointestinal motility in mice(P＞0.05). The anesthetic-dose and low-dose of sodium oxybate[ (48±5)％ and (52±6)％] decreased the rate of gastrointestinal motility(P＜0.05). Conclusion The 2％ dose of formalin significantly reduced the rate of gastrointestinal motility in mice. The anesthesia-dose ketamine promoted the gastrointestinal motility in formalin-induced pain model mice, while the anesthetic-dose and low-dose of sodium oxybate could inhibit the gastrointestinal motility in mice.