国际医学寄生虫病杂志
國際醫學寄生蟲病雜誌
국제의학기생충병잡지
INTERNATIONAL JOURNAL OF MEDICAL PARASITIC DISEASES
2011年
1期
23-27
,共5页
关飞%牛安欧%韩庆霞%陈琼荣
關飛%牛安歐%韓慶霞%陳瓊榮
관비%우안구%한경하%진경영
湖北钉螺%细胞色素氧化酶Ⅰ%系统发育
湖北釘螺%細胞色素氧化酶Ⅰ%繫統髮育
호북정라%세포색소양화매Ⅰ%계통발육
Oncomelania hupensis%Cytochrome oxidase Ⅰ%Phylogeny
目的 研究中国21株湖北钉螺细胞色素氧化酶Ⅰ(cytochrome oxidase Ⅰ,CO Ⅰ)基因序列差异和各地域株的亚种分化.方法 收集中国4省7地湖北钉螺标本,提取基因组DNA后,PCR扩增CO Ⅰ基因部分片段并进行序列测定.加入原有14株钉螺CO Ⅰ序列,用Clustal X进行序列比对后输入MEGA软件计算遗传距离,并分析序列集变异情况.采用距离法构建系统进化树.结果 所有21株标本经过序列比对得到一致性长度为611 bp.序列集中变异位点为19.3%.编码的203个氨基酸中仅有1个氨基酸的差异.21株钉螺平均遗传距离为0.0526,其中四川、云南株与其他地域株之间差异较大,为0.1296～0.1572,其余两两之间的距离均＜0.06.采用邻接法和非加权组平均法两种方法构建系统进化树,拓扑结构基本一致,四川、云南株聚为一支,其余株位于另外分支,且湖北赤壁株及湖北远安、钟祥株显示出特异性.结论 中国湖北钉螺CO Ⅰ基因存在差异.原有分类中滇川、福建、指名、广西亚种成立.湖北赤壁株似为独立亚种.
目的 研究中國21株湖北釘螺細胞色素氧化酶Ⅰ(cytochrome oxidase Ⅰ,CO Ⅰ)基因序列差異和各地域株的亞種分化.方法 收集中國4省7地湖北釘螺標本,提取基因組DNA後,PCR擴增CO Ⅰ基因部分片段併進行序列測定.加入原有14株釘螺CO Ⅰ序列,用Clustal X進行序列比對後輸入MEGA軟件計算遺傳距離,併分析序列集變異情況.採用距離法構建繫統進化樹.結果 所有21株標本經過序列比對得到一緻性長度為611 bp.序列集中變異位點為19.3%.編碼的203箇氨基痠中僅有1箇氨基痠的差異.21株釘螺平均遺傳距離為0.0526,其中四川、雲南株與其他地域株之間差異較大,為0.1296～0.1572,其餘兩兩之間的距離均＜0.06.採用鄰接法和非加權組平均法兩種方法構建繫統進化樹,拓撲結構基本一緻,四川、雲南株聚為一支,其餘株位于另外分支,且湖北赤壁株及湖北遠安、鐘祥株顯示齣特異性.結論 中國湖北釘螺CO Ⅰ基因存在差異.原有分類中滇川、福建、指名、廣西亞種成立.湖北赤壁株似為獨立亞種.
목적 연구중국21주호북정라세포색소양화매Ⅰ(cytochrome oxidase Ⅰ,CO Ⅰ)기인서렬차이화각지역주적아충분화.방법 수집중국4성7지호북정라표본,제취기인조DNA후,PCR확증CO Ⅰ기인부분편단병진행서렬측정.가입원유14주정라CO Ⅰ서렬,용Clustal X진행서렬비대후수입MEGA연건계산유전거리,병분석서렬집변이정황.채용거리법구건계통진화수.결과 소유21주표본경과서렬비대득도일치성장도위611 bp.서렬집중변이위점위19.3%.편마적203개안기산중부유1개안기산적차이.21주정라평균유전거리위0.0526,기중사천、운남주여기타지역주지간차이교대,위0.1296～0.1572,기여량량지간적거리균＜0.06.채용린접법화비가권조평균법량충방법구건계통진화수,탁복결구기본일치,사천、운남주취위일지,기여주위우령외분지,차호북적벽주급호북원안、종상주현시출특이성.결론 중국호북정라CO Ⅰ기인존재차이.원유분류중전천、복건、지명、엄서아충성립.호북적벽주사위독립아충.
Objective To study the cytochrome oxidase Ⅰ sequence difference and subspecies evolution of 21 strains of Oncomelania hupensis in China. Methods Samples were collected from 7 sites in 4 provinces. Genomic DNAs were extracted from each strain and then used as template for CO Ⅰ fragment amplification by PCR. The amplified product was sequenced with primers in two directions respectively. The 7 sequences, together with other 14 CO Ⅰ sequences were aligned with Clustal X and then pair-wise distances were accounted using MEGA software. Phylogenetic trees were reconstructed based on distance methods. Results The CO Ⅰ sequences of all 21 stains had a length of 611 bp after a global alignment, 118 bp ( 19.3% ) of which were variable sites. The DNA sequence was translated into 203 amino acids with 1 different amino acid.Among the 21 strains, mean evolutional distance was 0.0526. The distance between Sichuan, Yunnan and other strains was 0. 1296～0. 1572, much more than others. The phylogenetic trees constructed with neighbourjoining(NJ) and unweighted pair group method with arithmetic mean(UPGMA) showed similar topological structure, strains of Sichuan and Yunnan clustered in one clade, while others were in another clade. There were some differences among the strains of Chibi, Yuan'an and Zhongxiang from Hubei Province. Conclusion The CO Ⅰ sequences of O. hupensis in China showed significant variation. Hubei-Chibi seemed to be an independent subspecies, except 4 existent subspecies of O. hupensis robertsoni, O. hupensis guangxiensis, O. hupensis tangi, O. hupensis hupensis.
