CAJ | 학술논문

目的研究新型不含汞的牙体充填材料镓合金的细胞毒性。方法采用一种能快速评定细胞增殖率和细胞毒性的比色分析方法——MTT比色法，检测镓合金和高铜银汞合金对小鼠成纤维细胞L929细胞相对增殖率的影响，评价其细胞毒性。结果参照上海市医用生物材料生物性能测试标准，镓合金表现出较高的细胞相对增殖率，2 d时为93.0%±4.9%,4 d时为102.0%±3.5%,7 d时为107.2%±4.2%, 其细胞毒性为0级，即无明显细胞毒性；高铜银汞合金表现为中度细胞毒性。结论镓合金无明显的细胞毒性。
목적연구신형불함홍적아체충전재료가합금적세포독성。방법채용일충능쾌속평정세포증식솔화세포독성적비색분석방법——MTT비색법，검측가합금화고동은홍합금대소서성섬유세포L929세포상대증식솔적영향，평개기세포독성。결과삼조상해시의용생물재료생물성능측시표준，가합금표현출교고적세포상대증식솔，2 d시위93.0%±4.9%,4 d시위102.0%±3.5%,7 d시위107.2%±4.2%, 기세포독성위0급，즉무명현세포독성；고동은홍합금표현위중도세포독성。결론가합금무명현적세포독성。
Objective　To test the cytotoxicity of Gallium alloy, a new mercury-free dental restorative material. Methods　L-929 mouse fibroblasts were used to detect the cell relative proliferation rate of Gallium alloy and high-copper amalgam by MTT-assay. Results　The results indecated that the relative growth rate induced by Gallium alloy was high (93.0%±4.9% 2ds, 102.0%±3.5% 4ds, 107.2%±4.2% 7ds), and the cytotoxicity of Gallium alloy was 0 grade according to the Test Standard of Shanghai Medical Biomaterial, meaning Gallium alloy had no significant cytotoxicity and the relative growth rate of Gallium alloy was higher than that of high-copper amalgam.The high-copper amalgam showed medium cytotoxicity. Conclusion　Gallium alloy has no significant cytotoxicity.