CAJ | 학술논문

目的探讨肠道病毒感染与云南省暴发性心肌炎病因学关系。方法选用新建立的免疫组织化学方法对云南省死于暴发性心肌炎及非感染性疾病患者心肌组织进行肠道病毒抗原原位检测，同时用相同方法检测患者心肌组织中是否存在腺病毒、巨细胞病毒、乙型及丙型肝炎病毒。结果 10例死于心肌炎暴发流行期及2例阳性对照患者心肌组织中均发现肠道病毒抗原存在，但死于其他疾病者未发现肠道病毒抗原，患者心肌组织中亦无其它致心肌炎病毒抗原存在。结论研究结果支持肠道病毒感染为云南省暴发致死性心肌炎发病病因。
목적탐토장도병독감염여운남성폭발성심기염병인학관계。방법선용신건립적면역조직화학방법대운남성사우폭발성심기염급비감염성질병환자심기조직진행장도병독항원원위검측，동시용상동방법검측환자심기조직중시부존재선병독、거세포병독、을형급병형간염병독。결과 10례사우심기염폭발류행기급2례양성대조환자심기조직중균발현장도병독항원존재，단사우기타질병자미발현장도병독항원，환자심기조직중역무기타치심기염병독항원존재。결론연구결과지지장도병독감염위운남성폭발치사성심기염발병병인。
Objective　To establish the etiological relationship between enterovirus infection and outbreaks of fulminant myocarditis in Yannan Province.Methods　Myocardial tissue samples from 10 patients who died during myocarditis outbreaks from July to August in different years in the Yunnan Province were collected to detect enterovirus capsid protein VP1 by an improved immunohistochemical method.Two patients with known enterovirus myocarditis and 4 cases who died of amniotic fluid embolism or drug intoxication from Yunnan Provine were served as positive or negative controls respectively.Tissue samples were also screened for antigens of adenoviruses,cytomegalovirus,hepatitis B and C vriruses.Results　All of 10 cases with fulminant myocarditis were positive for enterovirus VP1.The positive signal was mainly localized in the cytoplasm of focal or sattered myocytes.Enterovirus VP1 was also positive in the 2 positive controls.No enterovirus VP1 staining was found in negative controls.Adenovirus,cytomegalovirus,hepatitis B or C viruses were not found in any of these cases.Conclusions　The data of the present study suggest that enterovirus be the etiological agent of the outbreak of fulminat myocarditis in Yunnan Province.