CAJ | 학술논문

目的探讨H2S在心肺复苏后脑损伤中的作用及其可能机制.方法取SD雄性大鼠45只,随机(随机数字法)分为:(1)假手术组(A组)5只；(2)复苏组(B组),按自主循环恢复(restoration of spontaneous circulation,ROSC)后6、12、24、72 h分四个亚组,各5只；NaHS处理组(C组),亚组同B组.分别同时测定脑组织含水量、大鼠脑皮质H2S含量,免疫组化计数海马区巢蛋白(Nestin)阳性细胞并进行神经功能缺损评分(neurodeficit score,NDS).结果 B组中脑组织H2S含量呈先上升后下降的变化,72 h恢复正常水平.与B组比较,C组的脑组织含水量减少(P＜0.05或P＜0.01),Nestin表达增加(P＜0.05或P＜0.01).大鼠神经功能缺损评分改善(P＜0.05或P＜0.01),苏木精-伊红(HE)染色表明C组中脑组织海马区病理损伤轻于B组.结论内源性H2S可能参与心肺复苏后脑损伤的发生发展过程,小剂量外源性H2S可通过降低脑组织含水量,增加海马区Nestin的表达,发挥脑保护作用.
목적 탐토H2S재심폐복소후뇌손상중적작용급기가능궤제.방법 취SD웅성대서45지,수궤(수궤수자법)분위:(1)가수술조(A조)5지；(2)복소조(B조),안자주순배회복(restoration of spontaneous circulation,ROSC)후6、12、24、72 h분사개아조,각5지；NaHS처리조(C조),아조동B조.분별동시측정뇌조직함수량、대서뇌피질H2S함량,면역조화계수해마구소단백(Nestin)양성세포병진행신경공능결손평분(neurodeficit score,NDS).결과 B조중뇌조직H2S함량정선상승후하강적변화,72 h회복정상수평.여B조비교,C조적뇌조직함수량감소(P＜0.05혹P＜0.01),Nestin표체증가(P＜0.05혹P＜0.01).대서신경공능결손평분개선(P＜0.05혹P＜0.01),소목정-이홍(HE)염색표명C조중뇌조직해마구병리손상경우B조.결론 내원성H2S가능삼여심폐복소후뇌손상적발생발전과정,소제량외원성H2S가통과강저뇌조직함수량,증가해마구Nestin적표체,발휘뇌보호작용.
Objective To explore the effects of H2S on cerebral injury after cardiopulmonary resuscitation (CPR) and its mechanism.Methods Forty-five healthy Sprague-Dawley (SD) rats were randomly (random number) divided into shame-operated group ( group A,n =5 ),resuscitation group ( group B,further divided into four subgroups as per rats sacrificed 6 h,12 h,24 h,and 72 h after resuscitation,n =5),and NaHS pretreatment group ( group C,further divided into 4 subgroups as done in group B).The ratio of water content in brain tissue was calculated.The content of H2S in cerebral cortex of rats in all groups was determined by using universal microplate reader. Immunohistochemistry method was used to count the Nestin-positive cells. Results The content of H2S in hippocampus area of brain showed dramatic changes from rising up at first and then to lowering down to the minimum and finally returning to the original level in 72 h in B group.Compare to group B,brain water content was lesser ( P ＜0.05 or P ＜ 0.01 ) and the levels of Nestin in hippocampus increased in group C(P＜0.05 or P ＜0.01).The neurological deficit score (NDS) was improved (P ＜0.05 or P ＜0.01) and pathological changes in hippocampus of rat brain detected by using hemotoxylin - eosin staining were slighter in group C in comparison with group B.Conclusions Endogenous H2S may involve in the course of formation and progress of cerebral injury after CPR and small dose of NaHS (exogenous H2S) can improve NDS by decreasing cerebral edema and up-regulating Nestin level in hippocampus of brain,playing a protection role in cerebral injury after CPR.