中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2011年
11期
1884-1886
,共3页
许峰峰%张继红%邓量%梁力建
許峰峰%張繼紅%鄧量%樑力建
허봉봉%장계홍%산량%량력건
肝细胞癌%三羟异黄酮%PTEN基因
肝細胞癌%三羥異黃酮%PTEN基因
간세포암%삼간이황동%PTEN기인
Hepatocellular carcinoma%Genistein%PTEN
目的 探讨PTEN基因表达变化在三羟异黄酮(genistein)抑制裸鼠移植入肝癌增长中的作用及其机制.方法 将移植入肝癌裸鼠分为2组,对照组腹腔注入含0.04％二甲基亚砜(DMSO)的RPMI 1640培养基每天0.05 ml/g,Genistein组腹腔注入genistein每天1 mg/kg,3周后观察肝癌增长,并应用同位素试剂盒检测肝癌组织三磷酸肌醇( IP3)含量,逆转录－聚合酶链反应(RT-PCR)分析癌组织PTEN mRNA表达,Western blot分析肝癌组织PTEN蛋白表达.结果 Genistein组肝癌体积和重量均显著低于对照组,其中体积为(12.6±11.6) mm3比(52.3±26.5) mm3,重量为(42.7±27.8) mg比(91.3±31.4) mg,IP3含量显著低于对照组,为(13.4±1.4) pmol/mg蛋白比(35.3±6.6)pmol/mg蛋白,PTEN mRNA表达显著高于对照组,灰度与面积之积的相对强度(RI)为0.81±0.24比0.36±0.09,PTEN蛋白表达显著高于对照组,RI值为3.14±0.13比1.08±0.15.结论 PTEN基因表达上调在Genistein抑制裸鼠移植人肝癌增长中发生一定作用,其机制可能与抑制磷酸肌醇通路信号转导、抑制IP3生成有关.
目的 探討PTEN基因錶達變化在三羥異黃酮(genistein)抑製裸鼠移植入肝癌增長中的作用及其機製.方法 將移植入肝癌裸鼠分為2組,對照組腹腔註入含0.04％二甲基亞砜(DMSO)的RPMI 1640培養基每天0.05 ml/g,Genistein組腹腔註入genistein每天1 mg/kg,3週後觀察肝癌增長,併應用同位素試劑盒檢測肝癌組織三燐痠肌醇( IP3)含量,逆轉錄－聚閤酶鏈反應(RT-PCR)分析癌組織PTEN mRNA錶達,Western blot分析肝癌組織PTEN蛋白錶達.結果 Genistein組肝癌體積和重量均顯著低于對照組,其中體積為(12.6±11.6) mm3比(52.3±26.5) mm3,重量為(42.7±27.8) mg比(91.3±31.4) mg,IP3含量顯著低于對照組,為(13.4±1.4) pmol/mg蛋白比(35.3±6.6)pmol/mg蛋白,PTEN mRNA錶達顯著高于對照組,灰度與麵積之積的相對彊度(RI)為0.81±0.24比0.36±0.09,PTEN蛋白錶達顯著高于對照組,RI值為3.14±0.13比1.08±0.15.結論 PTEN基因錶達上調在Genistein抑製裸鼠移植人肝癌增長中髮生一定作用,其機製可能與抑製燐痠肌醇通路信號轉導、抑製IP3生成有關.
목적 탐토PTEN기인표체변화재삼간이황동(genistein)억제라서이식입간암증장중적작용급기궤제.방법 장이식입간암라서분위2조,대조조복강주입함0.04％이갑기아풍(DMSO)적RPMI 1640배양기매천0.05 ml/g,Genistein조복강주입genistein매천1 mg/kg,3주후관찰간암증장,병응용동위소시제합검측간암조직삼린산기순( IP3)함량,역전록－취합매련반응(RT-PCR)분석암조직PTEN mRNA표체,Western blot분석간암조직PTEN단백표체.결과 Genistein조간암체적화중량균현저저우대조조,기중체적위(12.6±11.6) mm3비(52.3±26.5) mm3,중량위(42.7±27.8) mg비(91.3±31.4) mg,IP3함량현저저우대조조,위(13.4±1.4) pmol/mg단백비(35.3±6.6)pmol/mg단백,PTEN mRNA표체현저고우대조조,회도여면적지적적상대강도(RI)위0.81±0.24비0.36±0.09,PTEN단백표체현저고우대조조,RI치위3.14±0.13비1.08±0.15.결론 PTEN기인표체상조재Genistein억제라서이식인간암증장중발생일정작용,기궤제가능여억제린산기순통로신호전도、억제IP3생성유관.
Objective To probe into the Role and mechanism of phosphatase and tensin homology deleted on chromosome ten (PTEN) gene expression change in inhabiting hepatocellular carcinoma of nude mice by genistein.Methods Animals with hepatocellular carcinoma were treated with genistein 1 mg/kgeveryday(ip) for 3 weeks,the volume and weight of tumaor was measured,Trisphosphate Inositol ( IP3 ),PTEN mRNA,PTEN protein were assayed by IP3-[ 3H] Birtrak assay,reverse transcription-polymerase chain reaction (RT-PCR),Western blotting,respectively.Results The tumor volume and weight of animals treated with genistein was lower than control [ Volume:( 12.6 ± 11.6) mm3 vs.(52.3 ± 26.5 )mm3,Weight:(42.7 ±27.8) mg vs.(91.3 ±31.4) mg],IP3 continent was lower than that of control [ ( 13.4± 1.4) pmol/mg protein vs.( 35.3 ± 6.6) pmol/mg protein),PTEN mRNA expression was higher in group treated with genistein than that of control,the RI which was the gray degree multiply area of PTEN/the gray degree multiply area of β-actin was 0.81 ± 0.24 vs.0.36 ± 0.09,PTEN mRNA expression was higher in group treated with genistein than that of control(3.14 ±0.13 vs.1.08±0.15).Condusion Genistein can inhabit growth of hepatocellular carcinoma tansplanted into liver of nude mice by up-regulating PTEN gene expression which is relative to signal transduction of phosphate Inositol leading to reduce IP3 production.
