中华实验和临床病毒学杂志
中華實驗和臨床病毒學雜誌
중화실험화림상병독학잡지
CHINESE JOURNAL OF EXPERIMENTAL AND CLINICAL VIROLOGY
2010年
6期
442-444
,共3页
梁红霞%陈媛媛%周荣%张倩%潘延凤%谷军生%李娟%江河清%余祖江
樑紅霞%陳媛媛%週榮%張倩%潘延鳳%穀軍生%李娟%江河清%餘祖江
량홍하%진원원%주영%장천%반연봉%곡군생%리연%강하청%여조강
HIV%肝炎病毒,乙型%感染控制%聚合酶链反应
HIV%肝炎病毒,乙型%感染控製%聚閤酶鏈反應
HIV%간염병독,을형%감염공제%취합매련반응
HIV%Hepatitis B virus%Infection%Polymerase chain reaction
目的 调查分析某艾滋病治疗示范区人免疫缺陷病毒(HIV)-1感染者中隐匿性乙型肝炎病毒(HBV)感染的情况及其影响因素.方法 采集某艾滋病治疗示范区97例经血感染HIV-1的感染者的血浆,采用酶联免疫吸附试验(ELISA)检测乙型肝炎表面抗原与抗体(HBsAg与抗HBs)、乙型肝炎e抗原与抗体(HBeAg与抗Hbe)、乙型肝炎核心抗体(抗HBc)及丙型肝炎抗体(抗HCV);采用吸附柱法抽提HBV DNA;采用巢式聚合酶链反应(PCR)法检测HBV S区;采用流式细胞仪计数CD4+T淋巴细胞.HBsAg阴性PCR阳性结果 者为合并隐匿性HBV感染者.合并隐匿性HBV感染者为实验组,未合并隐匿性HBV感染者为对照组.结果 97例HIV感染者中HBsAg阴性者92例(94.85%).92例HBsAg阴性者中合并隐匿性HBV感染者27例(29.35%),抗HCV阳性者73例(79.35%).合并隐匿性HBV感染者和未合并HBV感染者CD4+T淋巴细胞数、单独抗HBc阳性率分别为(212.11±133.1)和(318.9±172.2)cells/mm3、62.96%和18.46%,以上两指标两组比较差异均有统计学意义(P<0.01),两组间年龄、性别、是否合并HCV感染及抗HBs阳性率比较差异无统计学意义(P>0.05).结论 经有偿献血途径感染HIV者中存在隐匿性HBV感染;HIV阳性合并隐匿性HBV感染者中易出现单纯抗HBc阳性;CD4+T淋巴细胞数低的HIV感染者更容易合并隐匿性HBV感染.
目的 調查分析某艾滋病治療示範區人免疫缺陷病毒(HIV)-1感染者中隱匿性乙型肝炎病毒(HBV)感染的情況及其影響因素.方法 採集某艾滋病治療示範區97例經血感染HIV-1的感染者的血漿,採用酶聯免疫吸附試驗(ELISA)檢測乙型肝炎錶麵抗原與抗體(HBsAg與抗HBs)、乙型肝炎e抗原與抗體(HBeAg與抗Hbe)、乙型肝炎覈心抗體(抗HBc)及丙型肝炎抗體(抗HCV);採用吸附柱法抽提HBV DNA;採用巢式聚閤酶鏈反應(PCR)法檢測HBV S區;採用流式細胞儀計數CD4+T淋巴細胞.HBsAg陰性PCR暘性結果 者為閤併隱匿性HBV感染者.閤併隱匿性HBV感染者為實驗組,未閤併隱匿性HBV感染者為對照組.結果 97例HIV感染者中HBsAg陰性者92例(94.85%).92例HBsAg陰性者中閤併隱匿性HBV感染者27例(29.35%),抗HCV暘性者73例(79.35%).閤併隱匿性HBV感染者和未閤併HBV感染者CD4+T淋巴細胞數、單獨抗HBc暘性率分彆為(212.11±133.1)和(318.9±172.2)cells/mm3、62.96%和18.46%,以上兩指標兩組比較差異均有統計學意義(P<0.01),兩組間年齡、性彆、是否閤併HCV感染及抗HBs暘性率比較差異無統計學意義(P>0.05).結論 經有償獻血途徑感染HIV者中存在隱匿性HBV感染;HIV暘性閤併隱匿性HBV感染者中易齣現單純抗HBc暘性;CD4+T淋巴細胞數低的HIV感染者更容易閤併隱匿性HBV感染.
목적 조사분석모애자병치료시범구인면역결함병독(HIV)-1감염자중은닉성을형간염병독(HBV)감염적정황급기영향인소.방법 채집모애자병치료시범구97례경혈감염HIV-1적감염자적혈장,채용매련면역흡부시험(ELISA)검측을형간염표면항원여항체(HBsAg여항HBs)、을형간염e항원여항체(HBeAg여항Hbe)、을형간염핵심항체(항HBc)급병형간염항체(항HCV);채용흡부주법추제HBV DNA;채용소식취합매련반응(PCR)법검측HBV S구;채용류식세포의계수CD4+T림파세포.HBsAg음성PCR양성결과 자위합병은닉성HBV감염자.합병은닉성HBV감염자위실험조,미합병은닉성HBV감염자위대조조.결과 97례HIV감염자중HBsAg음성자92례(94.85%).92례HBsAg음성자중합병은닉성HBV감염자27례(29.35%),항HCV양성자73례(79.35%).합병은닉성HBV감염자화미합병HBV감염자CD4+T림파세포수、단독항HBc양성솔분별위(212.11±133.1)화(318.9±172.2)cells/mm3、62.96%화18.46%,이상량지표량조비교차이균유통계학의의(P<0.01),량조간년령、성별、시부합병HCV감염급항HBs양성솔비교차이무통계학의의(P>0.05).결론 경유상헌혈도경감염HIV자중존재은닉성HBV감염;HIV양성합병은닉성HBV감염자중역출현단순항HBc양성;CD4+T림파세포수저적HIV감염자경용역합병은닉성HBV감염.
Objective To assess the prevalence of occult HBV infection in HIV-infected patients inacquired immune deficiency syndrome area. Methods Serum samples were obtained from 97 HIV-infected patients who transmitted by paid blood donation. ELISA was used to detect HBV erologic markers (HBsAg, Anti-HBs, HBeAg, anti-HBe and anti-HBc) and HCV antibody. Flow Cytometry were used to detect CD4 +T cell count. Nested PCR was used to amplify surface protein region of HBV DNA. Results Ninety two patients were HBsAg negative in the 97 HIV-infected patients(94. 85% ). Twenty seven patients were co-infected with occult hepatitis B virus infection in the 92 HBsAg negative patients (29. 35% ).Seventy three patients were co-infected with HCV in the 92 HBsAg negative patients(79.35% ). CD4 cell count of subjects with occult HBV infection were significantly lower (212. 11 ± 133. 1 cells/mm3 versus 318.9 ± 172.2 cells/mm3, respectively, P <0. 01 ). A significantly higher prevalence of isolated anti-HBc was observed in HIV-infected subjects co-infectionded with occult HBV infection [62. 96% ( 13 of 27 )versus 18.46% (15 of 65 ) , P < 0. 01]. No statistical significant association could be established between the age, sex and whether co-infected with HCV. Conclusion It is found that occult HBV infection did occurs in HIV-infected patients. Individuals co-infected with HIV and occult HBV infection are more likely to have isolated anti-HBc than subjects with HIV alone. Co-infection with HIV and occult HBV is more