中华肿瘤杂志
中華腫瘤雜誌
중화종류잡지
CHINESE JOURNAL OF ONCOLOGY
2012年
8期
592-595
,共4页
周尘飞%计骏%袁菲%于颖彦%刘炳亚%张俊%朱正纲
週塵飛%計駿%袁菲%于穎彥%劉炳亞%張俊%硃正綱
주진비%계준%원비%우영언%류병아%장준%주정강
胃肿瘤%转移相关基因2%核转录因子Sp1
胃腫瘤%轉移相關基因2%覈轉錄因子Sp1
위종류%전이상관기인2%핵전록인자Sp1
Gastric neoplasms%Metastasis associated 1 family member 2%Nuclear transcription factor Sp1
目的 观察转移相关基因2( MTA2)在胃癌组织中的表达特征,明确MTA2表达与核转录因子Sp1表达的相关性.方法 采用免疫组织化学染色技术(IHC)和逆转录-聚合酶链式反应(RT-PCR)法,检测83例手术切除的胃癌原发灶和对应癌旁胃黏膜组织中MTA2的表达情况.采用IHC方法检测上述标本中核转录因子Sp1的表达.结果 MTA2在胃癌组织中的阳性表达率为31.3％,明显高于癌旁胃黏膜组织(12.0％),差异有统计学意义(P＜0.01).MTA2阳性与胃癌的浸润深度相关(x2=5.677,P＜0.05),但与患者的性别、年龄、分化程度、Lauren分型、淋巴结转移、远处转移和TNM分期无明显相关.MTA2的表达与胃癌组织中核转录因子Sp1的表达有关(r =0.320,P＜0.05),MTA2在Sp1阳性表达的胃癌组织中的表达率显著高于Sp1阴性表达者(x2=9.565,P＜0.01).RT-PCR检测结果显示,在Sp1高表达的胃癌组织中,MTA2 mRNA表达水平升高.结论 MTA2在胃癌组织中的表达率高于癌旁胃黏膜组织,并与胃癌的浸润深度相关.MTA2在核转录因子Sp1阳性胃癌组织中呈高度表达,可能与Sp1的转录调控有关.
目的 觀察轉移相關基因2( MTA2)在胃癌組織中的錶達特徵,明確MTA2錶達與覈轉錄因子Sp1錶達的相關性.方法 採用免疫組織化學染色技術(IHC)和逆轉錄-聚閤酶鏈式反應(RT-PCR)法,檢測83例手術切除的胃癌原髮竈和對應癌徬胃黏膜組織中MTA2的錶達情況.採用IHC方法檢測上述標本中覈轉錄因子Sp1的錶達.結果 MTA2在胃癌組織中的暘性錶達率為31.3％,明顯高于癌徬胃黏膜組織(12.0％),差異有統計學意義(P＜0.01).MTA2暘性與胃癌的浸潤深度相關(x2=5.677,P＜0.05),但與患者的性彆、年齡、分化程度、Lauren分型、淋巴結轉移、遠處轉移和TNM分期無明顯相關.MTA2的錶達與胃癌組織中覈轉錄因子Sp1的錶達有關(r =0.320,P＜0.05),MTA2在Sp1暘性錶達的胃癌組織中的錶達率顯著高于Sp1陰性錶達者(x2=9.565,P＜0.01).RT-PCR檢測結果顯示,在Sp1高錶達的胃癌組織中,MTA2 mRNA錶達水平升高.結論 MTA2在胃癌組織中的錶達率高于癌徬胃黏膜組織,併與胃癌的浸潤深度相關.MTA2在覈轉錄因子Sp1暘性胃癌組織中呈高度錶達,可能與Sp1的轉錄調控有關.
목적 관찰전이상관기인2( MTA2)재위암조직중적표체특정,명학MTA2표체여핵전록인자Sp1표체적상관성.방법 채용면역조직화학염색기술(IHC)화역전록-취합매련식반응(RT-PCR)법,검측83례수술절제적위암원발조화대응암방위점막조직중MTA2적표체정황.채용IHC방법검측상술표본중핵전록인자Sp1적표체.결과 MTA2재위암조직중적양성표체솔위31.3％,명현고우암방위점막조직(12.0％),차이유통계학의의(P＜0.01).MTA2양성여위암적침윤심도상관(x2=5.677,P＜0.05),단여환자적성별、년령、분화정도、Lauren분형、림파결전이、원처전이화TNM분기무명현상관.MTA2적표체여위암조직중핵전록인자Sp1적표체유관(r =0.320,P＜0.05),MTA2재Sp1양성표체적위암조직중적표체솔현저고우Sp1음성표체자(x2=9.565,P＜0.01).RT-PCR검측결과현시,재Sp1고표체적위암조직중,MTA2 mRNA표체수평승고.결론 MTA2재위암조직중적표체솔고우암방위점막조직,병여위암적침윤심도상관.MTA2재핵전록인자Sp1양성위암조직중정고도표체,가능여Sp1적전록조공유관.
Objective To determine the expression patterns of metastasis associated 1 family member 2 (MTA2) in gastric cancer and non-cancerous gastric mucosa,and analyze its relationship with nuclear transcription factor Sp1 expression. Methods Tissue samples and clinicopathological information from 83 gastric cancer patients,who underwent surgery,were collected from Shanghai Rui Jin Hospital.All samples included cancer tissue and non-cancerous mucosa which was 5 cm away from the tumor lesion.The expression of MTA2 and Sp1 were detected by immunohistochemistry (IHC) staining.The mRNA of MTA2 was also detected by reverse transcription-polymerase chain reaction (RT-PCR).SPSS software was used for statistical analysis.Results The expression of MTA2 protein was significantly higher in primary lesions of the gastric cancer than that in non-cancerous mucosa by IHC (31.3％ vs 12.0％,P ＜0.01 ).MTA2 expression was closely related with tumor invasion or T staging (x2 =5.677,P ＜ 0.05 ).Yet,no significant relationship was observed between MTA2 expression and other clinicopathological parameters,including the age,sex,rumor differentiation,Lauren classification,lymph node metastasis,distant metastasis,as well as pathological staging.Furthermore,MTA2 expression was concomitant with Sp1 expression (r =0.320,P ＜0.05 ).Elevated MTA2 expression was observed in Sp1 positive cancer tissues (x2 =9.565,P ＜ 0.01 ).RT-PCR results also demonstrated that MTA2 mRNA was also highly expressed in the tissue samples with Sp1 expression.Conclusions MTA2 is highly expressed in the primary lesions of gastric cancer than that in adjacent non-cancerous tissues,and is closely related with tumor invasion.MTA2 expression is elevated in Sp1 positive gastric cancer.
