CAJ | 학술논문

应用胶体金免疫层析技术建立了恩诺沙星和环丙沙星快速检测试纸，并对试纸条的检测限、特异性和稳定性等参数进行了确定。结果表明，该试纸条对牛奶样本的检测限为50μg/L，能够特异性地检测恩诺沙星和环丙沙星，与液相色谱-串联质谱方法检测的阴性样本符合率为97．8％。该试纸条在2℃～8℃或室温可保存12个月，可应用于恩诺沙星在牛奶中的快速筛查现场检测。
응용효체금면역층석기술건립료은낙사성화배병사성쾌속검측시지，병대시지조적검측한、특이성화은정성등삼수진행료학정。결과표명，해시지조대우내양본적검측한위50μg/L，능구특이성지검측은낙사성화배병사성，여액상색보-천련질보방법검측적음성양본부합솔위97．8％。해시지조재2℃～8℃혹실온가보존12개월，가응용우은낙사성재우내중적쾌속사사현장검측。
The rapid test strip for detection of enrofloxacin and ciprofloxacin was developed based on colloidal gold immune chromatographic technology, and its technical parameters, such as sensitivity, specificity and stability were tested respectively. The results showed that the LOD of the strip was 50 μg/L and was specific for enrofloxacin and ciprofloxacin detection. The coincidence rate of negative samples was 97.8% compared with LC - MS/MS. The rapid test strip can be stored at 2 % - 8 ℃ or room temperature for at least 12 months and be applied for rapid enrofloxacin screening in milk.