CAJ | 학술논문

用野生杜鹃花的种子无茵发芽获得试管苗，然后用试管苗的叶片进行离体培养，用二步法建立杜鹃花的快繁体系。试验结果表明：诱导愈伤的最佳培养基是1／4Anderson＋2，4-D1mg／L＋TDZ0．1mg／L；芽诱导培养基是1／4 Anderson＋IBA0．1mg／L＋TDZ1mg／L＋2-IP0．5mg／L；生根培养基是1／4Anderson＋NAA0．1mg／L。
용야생두견화적충자무인발아획득시관묘，연후용시관묘적협편진행리체배양，용이보법건립두견화적쾌번체계。시험결과표명：유도유상적최가배양기시1／4Anderson＋2，4-D1mg／L＋TDZ0．1mg／L；아유도배양기시1／4 Anderson＋IBA0．1mg／L＋TDZ1mg／L＋2-IP0．5mg／L；생근배양기시1／4Anderson＋NAA0．1mg／L。
The seeds of Rhododendron simsii germinated under aseptic conditions , then the leaves from Rhododendron tube seedlings were used as explants , the system of rapid propagation was established by means of two steps method. The results were as follows; The best callus induction medium was 1/4Anderson ＋ 2, 4-D 1 mg/L ＋ TDZ 0.1 mg/L; the best shoots induction medium was 1/4Anderson ＋ IBA0.1 mg/L ＋ TDZ 1 mg/L ＋ 2-IP 0.5 mg/L; the best medium for rooting was 1/4Anderson ＋ NAA 0.1 mg/L.