CAJ | 학술논문

[目的]以替考拉宁产品本身为筛选剂,对替考拉宁产生菌替考游动放线菌（Actinoplanes teichomyceticus）进行了推理化选育,以便获得高产株。[方法]通过将亲株的孢子悬浮液用紫外线照射一定的时间后,涂布于含一定替考拉宁产品的分离培养基上,培养一定时间后挑选在上面生长的菌落进行发酵水平的测定。[结果]获得了比亲株发酵水平提高20%的菌株。传代试验表明,获得的菌株遗传稳定性较高。将其应用于50L自控罐进行发酵水平的验证,结果发酵水平比亲株提高30%以上。[结论]以替考拉宁产品本身为筛选剂进行推理化选育是切实可行的。
[목적]이체고랍저산품본신위사선제,대체고랍저산생균체고유동방선균（Actinoplanes teichomyceticus）진행료추이화선육,이편획득고산주。[방법]통과장친주적포자현부액용자외선조사일정적시간후,도포우함일정체고랍저산품적분리배양기상,배양일정시간후도선재상면생장적균락진행발효수평적측정。[결과]획득료비친주발효수평제고20%적균주。전대시험표명,획득적균주유전은정성교고。장기응용우50L자공관진행발효수평적험증,결과발효수평비친주제고30%이상。[결론]이체고랍저산품본신위사선제진행추이화선육시절실가행적。
[Objective] Taking teicoplanin product itself as the selective agent,rational screening was made on the producing strain of teicoplanin Actinoplanes teichomyceticus. [Method] The spore suspension of parent strains were irradiated with ultraviolet radiation for certain time and smeared on the isolation medium containing certain teicoplanin product. After culturing for certain time,the grown bacterial colony was selected and the fermentation level was determined. [Result] The fermentation level of the obtained strains was 20% higher than that of parent strains. The subculture test indicated that the obtained strains had higher hereditary stability. The application of the mutant strain in a 50 L fermentor showed a 30% higher fermentation level than parent strain. [Conclusion] It is feasible to make rational screening by using teicoplanin itself as selective agent.