CAJ | 학술논문

[目的]以盆栽控病效果良好的一株植物内生细菌为材料,通过对铁载体的研究,探索其对棉花枯萎病的拮抗机制。[方法]通过蔗糖-天冬氨酸（Modified Sugar-Aspartic Acid,MSA）平板初步判断菌株是否产铁载体及其荧光特性;根据特定波长下吸光度值测定各菌株在液体MSA培养基内产铁载体活性;不同铁离子浓度下,比较其铁载体对棉花枯萎病的抑制作用;结合形态、生理生化等特征对其进行初步鉴定。[结果]该内生细菌在MSA培养基中高产的荧光铁载体对棉花枯萎病原真菌——尖孢镰刀菌（Fusarium oxysporum）具有抑制作用,随着铁离子浓度的提高对病原真菌的抑制作用减弱;初步分析确定该内生菌株属于芽孢杆菌属（Bacillus）。[结论]芽孢杆菌可以通过分泌铁载体竞争铁的吸收而抑制尖孢镰刀菌的生长。
[목적]이분재공병효과량호적일주식물내생세균위재료,통과대철재체적연구,탐색기대면화고위병적길항궤제。[방법]통과자당-천동안산（Modified Sugar-Aspartic Acid,MSA）평판초보판단균주시부산철재체급기형광특성;근거특정파장하흡광도치측정각균주재액체MSA배양기내산철재체활성;불동철리자농도하,비교기철재체대면화고위병적억제작용;결합형태、생리생화등특정대기진행초보감정。[결과]해내생세균재MSA배양기중고산적형광철재체대면화고위병원진균——첨포렴도균（Fusarium oxysporum）구유억제작용,수착철리자농도적제고대병원진균적억제작용감약;초보분석학정해내생균주속우아포간균속（Bacillus）。[결론]아포간균가이통과분비철재체경쟁철적흡수이억제첨포렴도균적생장。
[Objective] With a strain of endophytic antagonistic bacteria with good disease control efficacy in pot as trial material,the paper was to explore the antagonistic mechanism of siderophore against Fusarium oxysporum.[Method] Whether the strain produced siderophore and its fluorescent property was judged by MSA（Modified Sugar-Aspartic acid） plate.The siderophore activity of strains in liquid MSA medium was determined through the absorbance values at particular wavelength.The inhibition effects of it siderophore against F.oxysporum under different concentrations of Fe3＋ were compared.Then the strain was preliminarily identified based on morphological,physiological and biochemical characteristics [Result] The fluorescent siderophore produced by the endophytic bacteria in MSA medium had a strong inhibition effect against F.oxysporum.With the increasing concentration of Fe3＋,the inhibition effect against the pathogen weakened.The preliminary analysis showed these strains belonged to Bacillus.[Conclusion] Bacillus could compete the absorption of Fe3＋ by secreting siderophore to inhibit the growth of F.oxysporum.