CAJ | 학술논문

目的：采用全血代替分离出的淋巴细胞作为实验对象,对于彗星实验方法中遇到的问题进行讨论。方法：选择大鼠作为实验动物,采用腹腔注射体内染毒24 h,尾部采血进行彗星实验。生理盐水染毒组为阴性对照组,环磷酰胺染毒组为阳性对照组（45μg/kg）。结果：全血彗星实验结果和分离淋巴细胞实验结果无显著差别（P〉0.05）;随着电泳条件中电压的升高,电泳时间的延长及裂解时间的延长,彗星尾长增长。用乙醇脱水法可保存彗星实验片子,与当天阅片结果无显著性差异。结论：微量全血彗星方法更简便,可用于大规模的筛选和检测工作。
목적：채용전혈대체분리출적림파세포작위실험대상,대우혜성실험방법중우도적문제진행토론。방법：선택대서작위실험동물,채용복강주사체내염독24 h,미부채혈진행혜성실험。생리염수염독조위음성대조조,배린선알염독조위양성대조조（45μg/kg）。결과：전혈혜성실험결과화분리림파세포실험결과무현저차별（P〉0.05）;수착전영조건중전압적승고,전영시간적연장급렬해시간적연장,혜성미장증장。용을순탈수법가보존혜성실험편자,여당천열편결과무현저성차이。결론：미량전혈혜성방법경간편,가용우대규모적사선화검측공작。
Objective： The problem of experimental method was discussed with whole blood and lymphocytes of rat by comet assay.Methods： Tail blood was collected to test by comet assay after 24 h intraperitoneal injection.Normal saline treated group used as the negative control.Cyclophosphamide treated group used as the positive control as treated（40 μg/kg）.Results： There was no marked difference between the result of blood comet and lymphocytes comet（P0.05）.With the rising of electrophoresis voltage and pyrolysis time,comet tail length increased.And there is no marked difference in result read on the same day and saved by ethanol dehydration.Conclusion： The results indicated that comet assay with trace quantities of whole blood can be used in screening.