CAJ | 학술논문

目的：了解HIV感染人群和健康人群口腔白色念珠菌基因型的分布情况。方法：运用CA-INT特异性引物聚合酶链反应方法扩增白色念珠菌包含1类内含子的编码rRNA的25S rDNA区,根据扩增条带大小进行基因分型,并应用χ2检验对54株HIV感染人群和54株健康人群口腔白色念珠菌基因型别进行分析。结果：CA-INT特异性引物PCR法可将108株口腔白色念珠菌分为A、B和C型,以A型最多见,两组人群口腔白色念珠菌基因型分布基本相同,但HIV感染人群口腔白色念珠菌A型所占构成比高于健康人群,差别有统计学意义。结论：HIV感染人群和健康人群口腔白色念珠菌均具有基因多态性,CA-INT特异性引物PCR法是较好的口腔白色念珠菌种内菌株间鉴定的方法。
목적：료해HIV감염인군화건강인군구강백색념주균기인형적분포정황。방법：운용CA-INT특이성인물취합매련반응방법확증백색념주균포함1류내함자적편마rRNA적25S rDNA구,근거확증조대대소진행기인분형,병응용χ2검험대54주HIV감염인군화54주건강인군구강백색념주균기인형별진행분석。결과：CA-INT특이성인물PCR법가장108주구강백색념주균분위A、B화C형,이A형최다견,량조인군구강백색념주균기인형분포기본상동,단HIV감염인군구강백색념주균A형소점구성비고우건강인군,차별유통계학의의。결론：HIV감염인군화건강인군구강백색념주균균구유기인다태성,CA-INT특이성인물PCR법시교호적구강백색념주균충내균주간감정적방법。
Objective： To investigate the distribution of genotypes of oral Candida albicans isolated from HIV-related group and healthy carriers group.Methods： PCR with CA-INT primers was designed to amplify group 1 intron-containing region in 25S rDNA of oral Candida albicans,the different strains of oral Candida albicans were classified into genotypes on the basis of bands from amplicons.Then the genotypic distribution results of all oral Candida albicans were analyzed with SPSS 17.0 by chi-square test.Results： One hundred and eight strains of oral cavities Candida albicans were classified into genotype A,B and C.Genotype A was the most predominant one.Identical genotypes distribution appeared in both groups.But the constituent ratio of genotype A of oral Candida albicans strains in HIV-positive group was statistically higher compared to the healthy people group.Conclusion： Genotypes of oral Candida albicans in both groups were highly polymorphic.The method in text is suitable to apply in genotyping research of oral Candida albicans.