昆明学院学报
昆明學院學報
곤명학원학보
JOURNAL OF KUNMING UNIVERSITY
2011年
3期
95-97
,共3页
帕罗西汀%立体选择性%高效液相色谱%对映体过量值
帕囉西汀%立體選擇性%高效液相色譜%對映體過量值
파라서정%입체선택성%고효액상색보%대영체과량치
Paroxetine%enantioselectivity%chiral high performance liquid chromatography%enantiomeric excess
通过手性柱高效液相色谱（HPLC）分析法,从实验室保藏菌种以及采集的土样中筛选出能立体选择性催化帕罗西汀中间体的产脂肪酶的微生物菌株.其中,编号为油4的菌株所产的脂肪酶具有相对较高的催化活力和立体选择性,在反应温度为35℃,反应时间为14 h时,催化水解化反应的底物转化率为39.64%,产物对映体过量值为17.8%.
通過手性柱高效液相色譜（HPLC）分析法,從實驗室保藏菌種以及採集的土樣中篩選齣能立體選擇性催化帕囉西汀中間體的產脂肪酶的微生物菌株.其中,編號為油4的菌株所產的脂肪酶具有相對較高的催化活力和立體選擇性,在反應溫度為35℃,反應時間為14 h時,催化水解化反應的底物轉化率為39.64%,產物對映體過量值為17.8%.
통과수성주고효액상색보（HPLC）분석법,종실험실보장균충이급채집적토양중사선출능입체선택성최화파라서정중간체적산지방매적미생물균주.기중,편호위유4적균주소산적지방매구유상대교고적최화활력화입체선택성,재반응온도위35℃,반응시간위14 h시,최화수해화반응적저물전화솔위39.64%,산물대영체과량치위17.8%.
Using chiral HPLC as an analytical method,Lipase-producing strains able to enantioselectivily catalyze Paroxetine intermediate were isolated from laboratory strains and soil samples,among which You4 displayed the highest enantiomeric excess value.The reaction conversion rate was 39.64%,and e.e.value of substrate was 17.8% when at 35℃ in 14h.Strain You4 was identified as bacillus through a series of physiological tests.
