CAJ | 학술논문

对中药火绒草乙醇提取物的正丁醇层的化学成分进行分离与鉴定。采用硅胶柱色谱法、葡聚糖凝胶LH-20柱色谱法和制备高效液相等方法进行分离纯化,并通过理化常数和谱学方法鉴定了其化学结构。从火绒草中分离得到了4个化合物,并鉴定了其结构（1-{3-（β-D-glucopyranosyloxy）-2,3-di-hydro-2-[1-（hydroxymethyl）vinyl]-1-benzofuan-5-yl}-ethanone 1）,小檗碱（berberin 2）,胡萝卜苷（daucosterol 3）,β-谷甾醇（β-sitosterol 4）。四个化合物分别为苯并呋喃类化合物1个,生物碱类化合物1个,甾体类化合物2个,其中化合物1首次从植物火绒草中分离得到。
대중약화융초을순제취물적정정순층적화학성분진행분리여감정。채용규효주색보법、포취당응효LH-20주색보법화제비고효액상등방법진행분리순화,병통과이화상수화보학방법감정료기화학결구。종화융초중분리득도료4개화합물,병감정료기결구（1-{3-（β-D-glucopyranosyloxy）-2,3-di-hydro-2-[1-（hydroxymethyl）vinyl]-1-benzofuan-5-yl}-ethanone 1）,소벽감（berberin 2）,호라복감（daucosterol 3）,β-곡치순（β-sitosterol 4）。사개화합물분별위분병부남류화합물1개,생물감류화합물1개,치체류화합물2개,기중화합물1수차종식물화융초중분리득도。
To study the chemical constituents of the n-BuOH extract part of the herbal Leontopodium leontopodioides（Wild.） Beauv.Ethanol extract.The compounds were isolated by chromatography on silica gel column,Sephadex LH-20 column chromatography and HPLC techniques,their structures were confirmed on the basis of spectral analysis.Four compounds were isolated and identified as 1-{3-（β-D-glucopyranosyloxy）-2,3-dihydro-2-[1-（hydroxyme-thyl）vinyl]-1-benzofuan-5-yl}-ethanone（1）,berberine（2）,daucosterol（3）,β-sitosterol（4）.Compound 1 was isolated from this herb firstly.