CAJ | 학술논문

目的:通过构建miR-181a的真核表达载体,研究其对人食管癌TE11细胞增殖、迁移和侵袭能力的影响.方法:以95C细胞基因组DNA为模板,扩增得到miR-181a前体序列,插入表达载体pcDNA3.1(+)克隆获得pcDNA3.1(+)-miR-181a.将真核表达载体pcDNA3.1(+)-miR-181a转染到TE11细胞中,并采用实时荧光定量-PCR(real-time fluorogentic quantitative-PCR,RFQPCR)对其表达情况进行验证.分别采用MTT法、细胞划痕法和Boyden小室法检测转染重组质粒pcDNA3.1(+)-miR-181a对TE11细胞增殖能力、迁移能力和侵袭能力的影响.结果:成功构建了插入miR-181a基因片段的真核表达载体pcDNA3.1(+)-miR-181a;RFO-PCR检测结果表明,转染pcDNA3.1(+)-miR-181a的TE11细胞能够过表达成熟的miR-181a(P<0.05).过表达miR-181a的TE11细胞增殖能力、迁移能力和细胞侵袭能力均明显增强.结论:miR-181a在TE11细胞中过表达能够增加细胞的增殖、迁移和侵袭能力,为进一步深入研究miR-181a在肿瘤中的作用机制提供了实验依据.
목적:통과구건miR-181a적진핵표체재체,연구기대인식관암TE11세포증식、천이화침습능력적영향.방법:이95C세포기인조DNA위모판,확증득도miR-181a전체서렬,삽입표체재체pcDNA3.1(+)극륭획득pcDNA3.1(+)-miR-181a.장진핵표체재체pcDNA3.1(+)-miR-181a전염도TE11세포중,병채용실시형광정량-PCR(real-time fluorogentic quantitative-PCR,RFQPCR)대기표체정황진행험증.분별채용MTT법、세포화흔법화Boyden소실법검측전염중조질립pcDNA3.1(+)-miR-181a대TE11세포증식능력、천이능력화침습능력적영향.결과:성공구건료삽입miR-181a기인편단적진핵표체재체pcDNA3.1(+)-miR-181a;RFO-PCR검측결과표명,전염pcDNA3.1(+)-miR-181a적TE11세포능구과표체성숙적miR-181a(P<0.05).과표체miR-181a적TE11세포증식능력、천이능력화세포침습능력균명현증강.결론:miR-181a재TE11세포중과표체능구증가세포적증식、천이화침습능력,위진일보심입연구miR-181a재종류중적작용궤제제공료실험의거.