CAJ | 학술논문

目的了解外源性肺表面活性物质（PS）对小肠缺血再灌注所致肺损伤的治疗效果。方法正常雄性SD大鼠27只，腹腔麻醉后随机分为3组（n＝9）,A组：钳夹肠系膜上动脉10s作为对照；B组：阻断动脉1h后，去除阻断，形成再灌注2h；C组：模型制作同B组，PS以100mg/kg气道滴入。所有动物再机械通气2h。监测动脉血气(PaO2)、肺顺应性（Cdyn）和气道阻力（R）和病理改变。结果血气分析B组PaO2进行性降低（B组与A组比较，P<0.05）,C组PS治疗后PaO2较为稳定,不表现为进行性下降,120min时较病变组差异有显著性意义（C组与B组比较，P<0.05），肺功能表现为B组Cdyn降低、R升高，治疗后Cdyn稳定，120min时略有升高（C组与B组比较，P<0.05），病理观察：B组肺水肿、间质出血、炎性细胞浸润及部分肺不张，C组治疗后病变减轻。结论外源性肺表面活性物质在这种模型的肺损伤治疗中可明显改善肺功能。
목적 료해외원성폐표면활성물질（PS）대소장결혈재관주소치폐손상적치료효과。방법 정상웅성SD대서27지，복강마취후수궤분위3조（n＝9）,A조：겸협장계막상동맥10s작위대조；B조：조단동맥1h후，거제조단，형성재관주2h；C조：모형제작동B조，PS이100mg/kg기도적입。소유동물재궤계통기2h。감측동맥혈기(PaO2)、폐순응성（Cdyn）화기도조력（R）화병리개변。결과 혈기분석B조PaO2진행성강저（B조여A조비교，P<0.05）,C조PS치료후PaO2교위은정,불표현위진행성하강,120min시교병변조차이유현저성의의（C조여B조비교，P<0.05），폐공능표현위B조Cdyn강저、R승고，치료후Cdyn은정，120min시략유승고（C조여B조비교，P<0.05），병리관찰：B조폐수종、간질출혈、염성세포침윤급부분폐불장，C조치료후병변감경。결론 외원성폐표면활성물질재저충모형적폐손상치료중가명현개선폐공능。
Objective To study the effects of exogenous pulmonarysurfactant(PS) on acute lung injury induced by intestinal ischemia-reperfusion in mice.Methods Twenty-seven healthy SD mice were anesthetized and divided equally into 3 groups at random, namely : Group A transient occlusion of mesenteric artery for 10 seconds as sham control; Group B occlusion of the artery for 1h followed by 2h reperfusion ; Group C intratracheal injection of PS (100mg/kg). All mice were tracheotomized and ventilated mechanically after the initial 3h, pH, dynamic compliance(Cdyn), and resistance(R) were measured simultaneously. Results 1. Pathological examination showed that the lung edema, hemorrhage and partial atelectasis were found in group B. All of these features were reversed by PS in group C;2. The gas exchange function was damaged in group B ( lower PaO2 and higher PaCO2, B vs A, P<0.05). After using PS, PaO2 was improved and PaCO2 decreased (C vs B, P<0.05); 3. The lung function of group B also showed decreasing in Cdyn and increasing in R. Cdyn in group C was obvious better than that in group B. Conclusion PS is an effective therapy in animal models of acute lung injury.