中国骨质疏松杂志
中國骨質疏鬆雜誌
중국골질소송잡지
CHINESE JOURNAL OF OSTEOPOROSIS
2010年
4期
274-278
,共5页
杨向东%余利鹏%韦永中%胡安全
楊嚮東%餘利鵬%韋永中%鬍安全
양향동%여리붕%위영중%호안전
β_2肾上腺素能受体激动剂%β_2肾上腺素能受体阻滞剂%破骨细胞%骨吸收陷窝
β_2腎上腺素能受體激動劑%β_2腎上腺素能受體阻滯劑%破骨細胞%骨吸收陷窩
β_2신상선소능수체격동제%β_2신상선소능수체조체제%파골세포%골흡수함와
β_2-adrenergic receptor-specific agonist%β_2-adrenergic receptor-specific antagonist%Osteoclast%Bone resorption
目的 研究选择性β_2肾上腺素能激动剂福莫特罗(Formoterol)和阻滞剂ICI118551对体外培养大鼠成熟破骨细胞(osteoclast,OC)功能的影响,探讨β_2肾上腺素能受体信号对骨代谢的影响.方法 取清洁级出生24 h内的SD乳大鼠,长骨干骨髓腔内壁机械分离成熟OC后分别加入不同浓度(10~(-5) mol/L～10~(-9) mol/L)的 Formoterol和ICI118551,以抗酒石酸酸性磷酸酶(TRAP)染色观察破骨细胞形态,甲苯胺蓝染色计数骨片上的骨吸收陷窝数目,Image-Pro Plus 6.0图像软件分析骨片上骨吸收陷窝面积.结果 破骨细胞与骨片共培养6天,不同浓度的Formoterol与对照组相比均可增加骨片上OC的骨吸收陷窝数目和面积;随着ICI118551浓度的提高骨片上骨吸收陷窝的数目和面积逐渐减少.结论 β_2肾上腺素能受体激动剂可促进体外培养OC的骨吸收功能,阻滞剂对OC的骨吸收功能有抑制作用,且呈剂量依赖性.
目的 研究選擇性β_2腎上腺素能激動劑福莫特囉(Formoterol)和阻滯劑ICI118551對體外培養大鼠成熟破骨細胞(osteoclast,OC)功能的影響,探討β_2腎上腺素能受體信號對骨代謝的影響.方法 取清潔級齣生24 h內的SD乳大鼠,長骨榦骨髓腔內壁機械分離成熟OC後分彆加入不同濃度(10~(-5) mol/L～10~(-9) mol/L)的 Formoterol和ICI118551,以抗酒石痠痠性燐痠酶(TRAP)染色觀察破骨細胞形態,甲苯胺藍染色計數骨片上的骨吸收陷窩數目,Image-Pro Plus 6.0圖像軟件分析骨片上骨吸收陷窩麵積.結果 破骨細胞與骨片共培養6天,不同濃度的Formoterol與對照組相比均可增加骨片上OC的骨吸收陷窩數目和麵積;隨著ICI118551濃度的提高骨片上骨吸收陷窩的數目和麵積逐漸減少.結論 β_2腎上腺素能受體激動劑可促進體外培養OC的骨吸收功能,阻滯劑對OC的骨吸收功能有抑製作用,且呈劑量依賴性.
목적 연구선택성β_2신상선소능격동제복막특라(Formoterol)화조체제ICI118551대체외배양대서성숙파골세포(osteoclast,OC)공능적영향,탐토β_2신상선소능수체신호대골대사적영향.방법 취청길급출생24 h내적SD유대서,장골간골수강내벽궤계분리성숙OC후분별가입불동농도(10~(-5) mol/L～10~(-9) mol/L)적 Formoterol화ICI118551,이항주석산산성린산매(TRAP)염색관찰파골세포형태,갑분알람염색계수골편상적골흡수함와수목,Image-Pro Plus 6.0도상연건분석골편상골흡수함와면적.결과 파골세포여골편공배양6천,불동농도적Formoterol여대조조상비균가증가골편상OC적골흡수함와수목화면적;수착ICI118551농도적제고골편상골흡수함와적수목화면적축점감소.결론 β_2신상선소능수체격동제가촉진체외배양OC적골흡수공능,조체제대OC적골흡수공능유억제작용,차정제량의뢰성.
Objective To evaluate the effects of Formoterol(β_2-adrenergic receptor-specific agonist) and ICI118551 (β_2-adrenergic receptor-specific antagonist) on rat matured ostoeclast cells in vitro.Methods Mature OC was isolated directly from neonatal 24-hour-SD rat long bones.The OC was treated with different concentrations of Formoterol and ICI118551 from 10~(-5) mol/L～10~(-9) mol/L respectively.Then the OC was stained with tartrate-resistant acid phosphatase (TRAP) to observe cellular morphology.The bone slices were stained with toluidine blue to count the number of the bone resorption pits and analyse the bone resorption pits by Image-Pro Plus 6.0.Results The number and area of the bone resorption pits increased in different concentrations of Formoterol treatment compared that in control treatment after osteoclasts and bone slices co-cultured for 6 days.Furthermore,the number and area of the bone resorption pits decreased with the dose of ICI118551 increased gradually.Conclusion The β_2-adrenergic receptor-specific agonist could promote the bone absorbion of OC in vitro.The β_2-adrenergic receptor-specific antagonist inhibits the bone resorption of OC in a dose-dependent manner.
