CAJ | 학술논문

用不同形态的镉培养液(氯化镉、硝酸镉、镉-EDTA、草酸镉和柠檬酸镉)对不同种类的蕨类植物(狗脊、舌厥、石松、瓦韦和石韦)进行培养.研究发现,镉在植物体不同部位的富集量是有差异的(富集量:根>茎>叶);对SEC-HPLC截取出的植物鳌合肽(PCs)研究表明,不间形态的镉能不同程度的诱发植物体中PCs的合成,从而PCs与Cd配位,降低镉的毒性,发现的配体主要为PC_3,iso-PC_3(βAla)和iso-PC_2(βAla).文章还研究了不同形态镉胁迫下的蕨类植物各部位中镉的分布情况,并且对植物体中经镉诱导形成的PCs-Cd配合物的形态进行研究,进而对其解毒机理进行探讨.
용불동형태적력배양액(록화력、초산력、력-EDTA、초산력화저몽산력)대불동충류적궐류식물(구척、설궐、석송、와위화석위)진행배양.연구발현,력재식물체불동부위적부집량시유차이적(부집량:근>경>협);대SEC-HPLC절취출적식물오합태(PCs)연구표명,불간형태적력능불동정도적유발식물체중PCs적합성,종이PCs여Cd배위,강저력적독성,발현적배체주요위PC_3,iso-PC_3(βAla)화iso-PC_2(βAla).문장환연구료불동형태력협박하적궐류식물각부위중력적분포정황,병차대식물체중경력유도형성적PCs-Cd배합물적형태진행연구,진이대기해독궤리진행탐토.
Different species of ferns (pyrrosia lingua, Lepisorus thunbergianus, Lycopodium japonicum, Elaphoglossum yoshina-gae and Woodwardia japonica) were cultivated using different species of cadmium as cultivation solution. The results showed that there were differences in enrichment amounts of cadmium in different parts of plants (amounts in root>in stem>in leaves). And it was found that different species of cadmium could induce synthesization of plant-chelated peptides (PCs) with different extents. And then PCs was coordinated with cadmium to decrease the toxicity from cadmium. There are some kinds of peptides ligands found in plants, which are mainly PC3, iso-PC3(βAla) and iso-PC2(βAla). In the present paper, the distribution of cad-mium in different parts of ferns was studied after the ferns were intimidated with different species of cadmium. And the species of PCs-Cd coordination compounds were also investigated to explain the detoxification mechanism aroused by it.