中国激光医学杂志
中國激光醫學雜誌
중국격광의학잡지
980235.htm
2009年
6期
345-349,401
,共6页
光动力疗法%鼻咽癌%CNE1细胞
光動力療法%鼻嚥癌%CNE1細胞
광동력요법%비인암%CNE1세포
Photodynamic therapy%Nasopharyngeal carcinoma%CNE1 cell%Apoptosis
目的 探讨新型酞菁类光敏剂锌酞菁H_1(ZnPcH_1)介导的光动力疗法(ZnPcH_1-PDT)对鼻咽癌CNE1细胞的杀伤作用.方法 采用人鼻咽癌CNE1细胞作为研究对象,应用MTF比色法和细胞集落形成实验检测PDT对CNE1细胞增殖的影响,采用AO/EB荧光染色法、TUNEL、DNA倍体分析检测细胞的死亡方式.结果 MTT比色法及细胞集落形成实验结果显示,ZnPcH_1-PDT可抑制鼻咽癌CNE1细胞增殖抑制率,并随ZnPcH_1的增加而增高(P<0.05);AO/EB荧光染色法、TUNNEL、DNA倍体分析结果均表明ZnPcH_1-PDT能够诱导CNE1细胞凋亡,凋亡率呈时间依赖性.结论 ZnPcH_1-PDT能够有效地抑制鼻咽癌CNE1细胞增殖,诱导该细胞凋亡,拥有良好的应用前景.
目的 探討新型酞菁類光敏劑鋅酞菁H_1(ZnPcH_1)介導的光動力療法(ZnPcH_1-PDT)對鼻嚥癌CNE1細胞的殺傷作用.方法 採用人鼻嚥癌CNE1細胞作為研究對象,應用MTF比色法和細胞集落形成實驗檢測PDT對CNE1細胞增殖的影響,採用AO/EB熒光染色法、TUNEL、DNA倍體分析檢測細胞的死亡方式.結果 MTT比色法及細胞集落形成實驗結果顯示,ZnPcH_1-PDT可抑製鼻嚥癌CNE1細胞增殖抑製率,併隨ZnPcH_1的增加而增高(P<0.05);AO/EB熒光染色法、TUNNEL、DNA倍體分析結果均錶明ZnPcH_1-PDT能夠誘導CNE1細胞凋亡,凋亡率呈時間依賴性.結論 ZnPcH_1-PDT能夠有效地抑製鼻嚥癌CNE1細胞增殖,誘導該細胞凋亡,擁有良好的應用前景.
목적 탐토신형태정류광민제자태정H_1(ZnPcH_1)개도적광동력요법(ZnPcH_1-PDT)대비인암CNE1세포적살상작용.방법 채용인비인암CNE1세포작위연구대상,응용MTF비색법화세포집락형성실험검측PDT대CNE1세포증식적영향,채용AO/EB형광염색법、TUNEL、DNA배체분석검측세포적사망방식.결과 MTT비색법급세포집락형성실험결과현시,ZnPcH_1-PDT가억제비인암CNE1세포증식억제솔,병수ZnPcH_1적증가이증고(P<0.05);AO/EB형광염색법、TUNNEL、DNA배체분석결과균표명ZnPcH_1-PDT능구유도CNE1세포조망,조망솔정시간의뢰성.결론 ZnPcH_1-PDT능구유효지억제비인암CNE1세포증식,유도해세포조망,옹유량호적응용전경.
Objective To investigate the killing effect of ZnPcH_1-PDT on the nasopharyngeal carcinoma cells.Methods The human nasopharyngeal carcinoma cell line CNE1 was selected to research.The survival rate of cells was assessed by MTT method and colony formation, the death model wag detected by AO/EB,TUNEL and cell cycle test.Results The results of MTT method and colony formation showed that ZnPcH_1-PDT both had significant killing effect on CNE1 cell.The results of AO/EB.TUNEL and cell cycle test showed that ZnPcH_1-PDT could induce apoptosis effectively .The apoptosis rate was increased in a time-dependent manner.Conclusions ZnPcH_1-PDT can inhibit nasopharyngeal carcinoma cells proliferation and induce apoptosis in a thne-dependment manner.
