CAJ | 학술논문

背景:髓核摘除术是治疗椎间盘突出的经典方法,但存在较高的复发率.目的:验证模拟人后路髓核摘除进行后外侧穿刺抽吸髓核,建立椎间盘退行性变动物模型的可行性.设计、时间及地点:观察对照实验,于2006-10/2007-02在解放军南京军区福州总医院动物实验室完成.材料:选用日本大耳白兔20只,行椎间盘退行性变动物模型制备.方法:用持针器夹持21G穿刺针,行L<,1-2>及L<,3-4>椎间盘右后外侧穿刺髓核抽吸法摘除部分髓核组织,术后2,4,8,12周分别对造模后椎闻盘行组织学观察,并将L<,2-3>椎间盘作为对照.主要观察指标:通过苏木精-伊红染色观察椎间盘组织学结构.结果:苏木精-伊红染色可见对照椎间盘大多髓核完整,髓核与纤维环分界清晰,纤维环结构接近正常,髓核组织中有大量髓核细胞.造模后第4周髓核细胞数量不断减少,第12周时髓核中主要为纤维组织,伴有极少量髓核细胞.结论:模拟人后路髓核摘除术建立后外侧纤维环穿刺髓核抽吸的椎间盘退行性变动物模型成功建立.可为应用组织工程修复重建退行性变椎间盘提供有效的动物模型.
배경:수핵적제술시치료추간반돌출적경전방법,단존재교고적복발솔.목적:험증모의인후로수핵적제진행후외측천자추흡수핵,건립추간반퇴행성변동물모형적가행성.설계、시간급지점:관찰대조실험,우2006-10/2007-02재해방군남경군구복주총의원동물실험실완성.재료:선용일본대이백토20지,행추간반퇴행성변동물모형제비.방법:용지침기협지21G천자침,행L<,1-2>급L<,3-4>추간반우후외측천자수핵추흡법적제부분수핵조직,술후2,4,8,12주분별대조모후추문반행조직학관찰,병장L<,2-3>추간반작위대조.주요관찰지표:통과소목정-이홍염색관찰추간반조직학결구.결과:소목정-이홍염색가견대조추간반대다수핵완정,수핵여섬유배분계청석,섬유배결구접근정상,수핵조직중유대량수핵세포.조모후제4주수핵세포수량불단감소,제12주시수핵중주요위섬유조직,반유겁소량수핵세포.결론:모의인후로수핵적제술건립후외측섬유배천자수핵추흡적추간반퇴행성변동물모형성공건립.가위응용조직공정수복중건퇴행성변추간반제공유효적동물모형.
BACKGROUND: Resection of the nudeus pulposus is the classical treatment for intervertebral disc protrusion, except a higher recurrence rate. OBJECTIVE: To verify the feasibility of establishing an animal model of intervertebral disc degeneration by puncture and aspiration via a posterolateral approach to simulate resection of human nucleus pulposus. DESIGN, TIME AND SE'I'FING: The experiment was conducted in the animal Experimental Center of Fuzhou General Hospital of Nanjing Military Area Command of Chinese PLA from October 2006 to February 2007. MATERIALS: Twenty Japanese big ear rabbits were selected to establish animal models of intervertebral disc degeneration. METHODS: Some nucleus pulposus tissues were abstracted from the L1-2 and L3-4 segment of 20 rabbits by the puncture and aspiration method using a 21-gaege hypodermic needle. Histological analysis was performed at 2, 4, 8, 12 weeks after surgery, and L2-3 segment was used as control group.MAIN OUTCOME MEASURE: Histological structure of the intervertebral disc was observed by homatoxylin-eosin staining. RESULTS: Hematoxylin-eosin staining revealed a great deal of complete nudeus pulposus tissues, clear boundaries between nucleus pulposus and annulus fibrosus in the control group, and the structure of near normal annulus fibrosus was almost normal, nucleus pulposus tissue had a large number of nucleus pulposus cells. In the experimental group, the nucleus pulposus cell reduced in amount in the fourth week, the nucleus pulposus at the twelfth week were mainly full of flbroblests, while few nucleus pulposus cells were found.CONCLUSION: It is successful to establishing an animal model of intervertebral disc degeneration by puncture and aspiration via a posterolateral approach based on simulating the resection of human nucleus pulposus. This model is available for repairing intervertebrai disc degeneration using tissue engineering techniques.