CAJ | 학술논문

目的：观察腺苷受体激动剂2-氯腺苷对大鼠脊髓损伤后神经功能和脊髓血流量的影响，探讨腺苷在继发性脊髓损伤中的作用。方法：脊髓腹侧压迫法造成T13脊髓损伤，用激光多普勒血流仪检测损伤前后的脊髓血流量，治疗组于伤前15min蛛网膜下腔注射不同剂量的非特异性腺苷受体激动剂2-氯腺苷（2-CADO），对照组注射同量的生理盐水。伤后24h观察神经功能评分、倾斜平面临界角和组织学变化。结果：脊髓损伤后脊髓血流量立即下降，而治疗组较对照组显著改善(P<0.05,P<0.01)。大剂量（500μmol/L）的2-CADO能显著改善损伤后的神经功能，而小剂量（50μmol/L）则对脊髓损伤无明显神经保护作用，大小剂量的作用有显著差异(P<0.01)。结论：腺苷通过改善脊髓血流量，从而对脊髓损伤起保护作用。
목적：관찰선감수체격동제2-록선감대대서척수손상후신경공능화척수혈류량적영향，탐토선감재계발성척수손상중적작용。방법：척수복측압박법조성T13척수손상，용격광다보륵혈류의검측손상전후적척수혈류량，치료조우상전15min주망막하강주사불동제량적비특이성선감수체격동제2-록선감（2-CADO），대조조주사동량적생리염수。상후24h관찰신경공능평분、경사평면림계각화조직학변화。결과：척수손상후척수혈류량립즉하강，이치료조교대조조현저개선(P<0.05,P<0.01)。대제량（500μmol/L）적2-CADO능현저개선손상후적신경공능，이소제량（50μmol/L）칙대척수손상무명현신경보호작용，대소제량적작용유현저차이(P<0.01)。결론：선감통과개선척수혈류량，종이대척수손상기보호작용。
Objective: To observe the effect of adenosine-receptor agonist2-chroloadenosine on spinal cord blood flow (SCBF) and neurological function after spinal cord injury(SCI) in rats, and to explore the mechanism of adenosine on SCI. Methods: SCI of T13 was made by ventral compression in rats. The SCBF was measured pre-injury and post-injury with laser Doppler flowmetry. In treatment group, different doses of 2-chroloadenosine (2-CADO) were given through intrathacal injection 15 min before injury. While in control group, the same volume of saline was given. The neurological function score, inclined plane angle and histology were observed 24 h after injury. Results: The SCBF decreased significantly after SCI. Treatment with 2-CADO improved SCBF significantly. High dose of 2-CADO （500 μmol/L） improved the neurological function of injuried rats significantly. But low dose of 2-CADO （50 μmol/L） showed no neuroprotective effect on SCI. Conclusion: Adenosine plays a neuroprotective role by improving SCBF in SCI rats.