微生物学通报
微生物學通報
미생물학통보
MICROBIOLOGY
2001年
2期
22-26
,共5页
蔡宝立%黄今勇%石建党%张心平%刘海%朱昌寿
蔡寶立%黃今勇%石建黨%張心平%劉海%硃昌壽
채보립%황금용%석건당%장심평%류해%주창수
阿特拉津%生物降解%菌株分离%阿特拉津氯水解酶基因
阿特拉津%生物降解%菌株分離%阿特拉津氯水解酶基因
아특랍진%생물강해%균주분리%아특랍진록수해매기인
从农药厂废水中分离到6株能以除草剂阿特拉津为唯一氮源生长的细菌，即假单胞菌(Pseu-domonas spp.)AD1、AD2和AD6，土壤杆菌(Agrobacterium sp.)AD4，黄单胞菌(Xanthomonas sp.)AD5，欧文氏菌(Erwinia sp.)AD7。AD1菌株能使无机盐培养基中的0.3g/L阿特拉津在72h内降解99.9％。当以AD1、AD2、AD4、AD5、AD6和AD7菌株的总DNA为模板进行PCR扩增时,除AD2菌株以外，均得到了与文献报道的假单胞菌ADP菌株的阿特拉津氯水解酶基因(atzA)同源的PCR产物。
從農藥廠廢水中分離到6株能以除草劑阿特拉津為唯一氮源生長的細菌，即假單胞菌(Pseu-domonas spp.)AD1、AD2和AD6，土壤桿菌(Agrobacterium sp.)AD4，黃單胞菌(Xanthomonas sp.)AD5，歐文氏菌(Erwinia sp.)AD7。AD1菌株能使無機鹽培養基中的0.3g/L阿特拉津在72h內降解99.9％。噹以AD1、AD2、AD4、AD5、AD6和AD7菌株的總DNA為模闆進行PCR擴增時,除AD2菌株以外，均得到瞭與文獻報道的假單胞菌ADP菌株的阿特拉津氯水解酶基因(atzA)同源的PCR產物。
종농약엄폐수중분리도6주능이제초제아특랍진위유일담원생장적세균，즉가단포균(Pseu-domonas spp.)AD1、AD2화AD6，토양간균(Agrobacterium sp.)AD4，황단포균(Xanthomonas sp.)AD5，구문씨균(Erwinia sp.)AD7。AD1균주능사무궤염배양기중적0.3g/L아특랍진재72h내강해99.9％。당이AD1、AD2、AD4、AD5、AD6화AD7균주적총DNA위모판진행PCR확증시,제AD2균주이외，균득도료여문헌보도적가단포균ADP균주적아특랍진록수해매기인(atzA)동원적PCR산물。
Six atrazine-degrading strains, Pseudomonas spp. AD1, AD2, AD6, Agrobacterium sp. AD4, Xanthomonas AD5, and Erwinia sp. AD7, were isolated from industrial wastewater. These strains are able to grow on atrazine as sole nitrogen source. Strain AD1 is able to degrade atrazine of 0. 3g/L in minimal medium at a percentage of 99.9% in 72 hours. PCR products that are homologous to the atrazine chlorohydrolase gene atzA)from Pseudomonas sp. strain ADP were obtained by PCR method using total DNA of the strains AD1 ,AD4,AD5,AD6,and AD7 as templates.
