CAJ | 학술논문

目的研究同型半胱氨酸(HCY)与脑梗塞的关系及叶酸、维生素B12(B12)对HCY水平的影响。方法采用高效液相色谱荧光检测法测定40例脑梗塞患者和20例同龄健康对照组血浆HCY浓度，用SimulTRAC-SNB放射法测定两组血清叶酸、B12的含量。结果①脑梗塞患者血浆HCY浓度(24.02*!μmol/L±11.24*!μmol/L)高于对照组(14.0*!μmol/L±4.48*!μmol/L)(P＜0.01)；②脑梗塞患者叶酸水平(4.82*!μg/L±1.97*!μg/L)显著低于对照组(6.88*!μg/L±2.85*!μg/L)(P＜0.001)；脑梗塞患者B12含量(247.56*!ng/L±158.72*!ng/L)略低于对照组(270.31*!ng/L±198.39*!ng/L)，但无显著差异(P＞0.05)，Pearson相关分析显示两组HCY浓度与叶酸水平均呈负相关性，与B12水平无显著相关。结论脑梗塞患者血浆HCY浓度高于同龄健康人；HCY浓度与叶酸水平呈负相关性，与B12水平相关性不显著。
목적연구동형반광안산(HCY)여뇌경새적관계급협산、유생소B12(B12)대HCY수평적영향。방법채용고효액상색보형광검측법측정40례뇌경새환자화20례동령건강대조조혈장HCY농도，용SimulTRAC-SNB방사법측정량조혈청협산、B12적함량。결과①뇌경새환자혈장HCY농도(24.02*!μmol/L±11.24*!μmol/L)고우대조조(14.0*!μmol/L±4.48*!μmol/L)(P＜0.01)；②뇌경새환자협산수평(4.82*!μg/L±1.97*!μg/L)현저저우대조조(6.88*!μg/L±2.85*!μg/L)(P＜0.001)；뇌경새환자B12함량(247.56*!ng/L±158.72*!ng/L)략저우대조조(270.31*!ng/L±198.39*!ng/L)，단무현저차이(P＞0.05)，Pearson상관분석현시량조HCY농도여협산수평균정부상관성，여B12수평무현저상관。결론뇌경새환자혈장HCY농도고우동령건강인；HCY농도여협산수평정부상관성，여B12수평상관성불현저。
To investigate the plasma homocysteine level and cerebral infarction and the association between plasma homocysteine level and levels of serum folate and vitamin B12.Methods　Levels of plasma homocysteine of 40 cerebral infarction patients and 20 healthy subjects were determined using high-performance liquid chromatography with fluorescence detection. Serum folate and vitamin B12concentrations were measured by SimulTRAC-SNB radioassay.Results　①The mean plasma level of homocystine was higher in patients than that in control［(24.02±11.24)*!μmol/L versus(14.10±4.48)*!μmol/L，P＜0.01］;②The level of serum folate was significantly lower in patients than that in control［(4.82±1.97)*!μg/L versus(6.88±2.85)*!μg/L,P＜0.001］.The level of serum B12was slightly lower in patients［(247.56±158.72)*!ng/L］ than that in control［(270.31±198.39)*!ng/L］,but the difference was not statistically significant (P＞0.05).Person correlation anslysis revealed that plasma homocysteine level showed a strong inverse association with folate level in patients(r=-0.39，P=0.01)and in controls(r=-0.46，P=0.03).Vitamin B12 did not show correlation to plasma homocysteine in cases or controls(P＞0.05).Conclusion　①Plasma homocysteine level in cerebral infarction patients is higher than that in the same age health.②There is a strong inverse association between plasma homocysteine and folate.The association between plasma homocysteine and vitamin B12 is not statistically significant.