CAJ | 학술논문

以小麦幼胚为基因枪转化受体,用含GUS和bar基因的质粒pDM803对小麦幼胚进行转化,并对幼胚大小、轰击距离|质粒和金粉用量等参数对GUS瞬时表达率及表达量的影响进行了研究.幼胚以1.0～1.5 mm最为适宜,6 cm和9 cm各轰击一次、质粒0.5 μg、枪及金粉300 μg、枪,GUS瞬时表达率及表达量最高.通过对以上参数的优化,建立了一个高效的小麦基因枪转化体系.
이소맥유배위기인창전화수체,용함GUS화bar기인적질립pDM803대소맥유배진행전화,병대유배대소、굉격거리|질립화금분용량등삼수대GUS순시표체솔급표체량적영향진행료연구.유배이1.0～1.5 mm최위괄의,6 cm화9 cm각굉격일차、질립0.5 μg、창급금분300 μg、창,GUS순시표체솔급표체량최고.통과대이상삼수적우화,건립료일개고효적소맥기인창전화체계.
An efficient transformation system for wheat was established by improving transformation conditions for the particle bombardment of immature embryos.Particle bombardment was carried out using pDM803 containing the reporter gene β-glucuronidase(GUS) and bar gene.Transformation parameters,such as the size of immature embryos,the distance of bombardment,the amount of gold particles and plasmid used per bombardment were analyzed.The immature embryo of 1.0～1.5 mm was optimal.The transient expression of GUS reached the highest when the immature embryos were bombarded twice at the distance of 6 cm and 9 cm,using 300 μg gold particles and 0.5 μg plasmid per bombardment.