落叶果树
落葉果樹
락협과수
DECIDUOUS FRUITS
2001年
2期
4-6
,共3页
吴雅琴%章德明%LU Qizhen
吳雅琴%章德明%LU Qizhen
오아금%장덕명%LU Qizhen
离体微嫁接法%苹果褪绿叶斑病毒%苹果茎沟病毒%苹果茎痘病毒%检测方法
離體微嫁接法%蘋果褪綠葉斑病毒%蘋果莖溝病毒%蘋果莖痘病毒%檢測方法
리체미가접법%평과퇴록협반병독%평과경구병독%평과경두병독%검측방법
用离体微嫁接法同时检测了苹果褪绿叶斑病毒、苹果茎沟病毒和苹果茎痘病毒。由于苹果褪绿叶斑病毒和苹果茎沟病毒,症状是在叶部表现黄斑,试管内不易观察,病毒检出率较低,检测带毒率分别为37.5%和61.1%,在检测苹果茎痘病毒上取得成功。检出率达81.3%以上。用木本指示植物跟踪检测了带毒品种,试验证明采用离体微嫁接法检测果树病毒比用木本指示植物检测所需时间短,病毒检出率基本一致。
用離體微嫁接法同時檢測瞭蘋果褪綠葉斑病毒、蘋果莖溝病毒和蘋果莖痘病毒。由于蘋果褪綠葉斑病毒和蘋果莖溝病毒,癥狀是在葉部錶現黃斑,試管內不易觀察,病毒檢齣率較低,檢測帶毒率分彆為37.5%和61.1%,在檢測蘋果莖痘病毒上取得成功。檢齣率達81.3%以上。用木本指示植物跟蹤檢測瞭帶毒品種,試驗證明採用離體微嫁接法檢測果樹病毒比用木本指示植物檢測所需時間短,病毒檢齣率基本一緻。
용리체미가접법동시검측료평과퇴록협반병독、평과경구병독화평과경두병독。유우평과퇴록협반병독화평과경구병독,증상시재협부표현황반,시관내불역관찰,병독검출솔교저,검측대독솔분별위37.5%화61.1%,재검측평과경두병독상취득성공。검출솔체81.3%이상。용목본지시식물근종검측료대독품충,시험증명채용리체미가접법검측과수병독비용목본지시식물검측소수시간단,병독검출솔기본일치。
Apple chlorotic leafspot virus(ACLSV), apple stem grooving virus (ASGV) and apple stem pitting virus (ASPV) were detected by in vitro micrografting. ACLSV and ASGV couldn't easy to be observed in the test tube, with the rates detected being 37.5 % for the former and 61 : 1% for the later, because their symptoms were yellow spots on the leaf of indicator plants. But it was succeded in detection of ASPV, with the rate of the virus detected being more than 81.3 %. Detection of virus - carrying varieties with woody indicators by tracing showed that use of micrografting in vitro required less time than use of wood indicators, but the rate of virus detected was the same on the whole.