分析化学
分析化學
분석화학
CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
2001年
2期
199-201
,共3页
徐桂芸%常溪燕%常理文
徐桂蕓%常溪燕%常理文
서계예%상계연%상리문
神经递质%脉冲安培检测%高效液相色谱
神經遞質%脈遲安培檢測%高效液相色譜
신경체질%맥충안배검측%고효액상색보
详细对比了脉冲安培与恒电位安培检测单胺类神经递质的响应值,结果表明脉冲安培检测上述物质的响应值是恒电位安培检测的1.2倍。建立了以3.4-二羟基苄胺(DHBA)为内标的定量方法。在微透析溶液中的微量神经递质的回收率在74%~101%之间。
詳細對比瞭脈遲安培與恆電位安培檢測單胺類神經遞質的響應值,結果錶明脈遲安培檢測上述物質的響應值是恆電位安培檢測的1.2倍。建立瞭以3.4-二羥基芐胺(DHBA)為內標的定量方法。在微透析溶液中的微量神經遞質的迴收率在74%~101%之間。
상세대비료맥충안배여항전위안배검측단알류신경체질적향응치,결과표명맥충안배검측상술물질적향응치시항전위안배검측적1.2배。건립료이3.4-이간기변알(DHBA)위내표적정량방법。재미투석용액중적미량신경체질적회수솔재74%~101%지간。
A method for determinattion of monoamine neurotransmitters and related compounds in microdialysis solution by high performance liquid chromatography (HPLC) with pulsed amperometric detection was described. A comparison of response of iso potential anperometric (AD) and pulsed amperometric detection (PAD) was made. The results indicated that the sensitivity of PAD was 1.2 fold that of AD. The quantitative determination of monoamine neurotransmitters and related compounds was carried out with 3,4-dihydroxybenzylamine (DHBA) as internal standerd. The recoveries of monoamine neurotransmitters in microdialysis solution are 71% ~ 101%.