中国神经免疫学和神经病学杂志
中國神經免疫學和神經病學雜誌
중국신경면역학화신경병학잡지
CHINESE JOURNAL OF NEUROIMMUNOLOGY AND
2001年
1期
52-55
,共4页
赵昕%韩靖云%李红%叶贤坤%赵振军
趙昕%韓靖雲%李紅%葉賢坤%趙振軍
조흔%한정운%리홍%협현곤%조진군
肌酸激酶亚型%假肥大型肌营养不良%电泳
肌痠激酶亞型%假肥大型肌營養不良%電泳
기산격매아형%가비대형기영양불량%전영
目的 研究假肥大型肌营养不良（DMD）患者肌型肌酸激酶（CK-MM）亚型的变化，为早期诊断和正确评价病情提供依据。方法 采用不连续缓冲体系，在稳流低压条件下电泳分离CK-MM亚型，荧光扫描。结果 随着DMD患者病情的加重，其不同阶段的MM2/MM1均与对照组差异显著（P＜0.05）；DMD患者不同阶段的MM2/MM1值差异也显著（P＜0.05）。结论 CK-MM亚型的改变是DMD的早期诊断指标，是判断病情及科学评价治疗效果的依据。
目的 研究假肥大型肌營養不良（DMD）患者肌型肌痠激酶（CK-MM）亞型的變化，為早期診斷和正確評價病情提供依據。方法 採用不連續緩遲體繫，在穩流低壓條件下電泳分離CK-MM亞型，熒光掃描。結果 隨著DMD患者病情的加重，其不同階段的MM2/MM1均與對照組差異顯著（P＜0.05）；DMD患者不同階段的MM2/MM1值差異也顯著（P＜0.05）。結論 CK-MM亞型的改變是DMD的早期診斷指標，是判斷病情及科學評價治療效果的依據。
목적 연구가비대형기영양불량（DMD）환자기형기산격매（CK-MM）아형적변화，위조기진단화정학평개병정제공의거。방법 채용불련속완충체계，재은류저압조건하전영분리CK-MM아형，형광소묘。결과 수착DMD환자병정적가중，기불동계단적MM2/MM1균여대조조차이현저（P＜0.05）；DMD환자불동계단적MM2/MM1치차이야현저（P＜0.05）。결론 CK-MM아형적개변시DMD적조기진단지표，시판단병정급과학평개치료효과적의거。
Objective To study the changes of isoforms of creatine kinaseCK-MM in patients with Duchenne muscular dystrophy for the purpose of early diagnosis as well as evaluation of seriousness of the disease. Methods A dis-continuous buffer system was used. CK-MM isoform was separated by electrophoresis under the condition of constant current and low voltage, then fluorescence scanning. Results The nature of DMD patient was gradually getting worse along with the age increase. There was a statistically significant change of the isoform ratio MM2/MM1 in each stage of DMD compared with the normal control ( P＜ 0.05 ); there was also significant change of the isoform ratio MM2/MM1 between each stage of the subject ( P ＜ 0.05 ). Conclusions The change of CK-MM isoforms is a specific indicator in early diagnosis of DMD. It is a important indicator for evaluation of seriousness of the disease. It is also useful in the evaluation of the real effect of some treatment methods.
