CAJ | 학술논문

汉坦病毒Z37株是从褐家鼠体内分离到的，用于生产双价肾综合征出血热疫苗的病毒毒株之一，血清分型为SEO型。利用RT-PCR方法扩增Z37株M基因片段cDNA，克隆入质粒载体，进行核苷酸序列测定及分析。Z37株M基因片段由3651个核苷酸组成，只有一个开放读码框架，共编码1133个氨基酸。与HTN型病毒(76-118、A9、HV-114)的核苷酸和氨基酸同源性分别为71.8%～72.1%、76.2%～76.7%，与SEO型(R22、L99、80-39)的核苷酸和氨基酸同源性分别为95.3%～96.1%、95.3%～98.9%。这一结果的获得进一步从分子水平确定了Z37株的型别，并为研制M基因片段重组疫苗打下基础。
한탄병독Z37주시종갈가서체내분리도적，용우생산쌍개신종합정출혈열역묘적병독독주지일，혈청분형위SEO형。이용RT-PCR방법확증Z37주M기인편단cDNA， 극륭입질립재체，진행핵감산서렬측정급분석。Z37주M기인편단유3651개핵감산조성，지유 일개개방독마광가，공편마1133개안기산。여HTN형병독(76-118、A9、HV-114)적핵감산 화안기산동원성분별위71.8%～72.1%、76.2%～76.7%，여SEO형(R22、L99、80-39)적핵감 산화안기산동원성분별위95.3%～96.1%、95.3%～98.9%。저일결과적획득진일보종분자수평 학정료Z37주적형별，병위연제M기인편단중조역묘타하기출。
One of the strain of bivalent HFRS vaccine, Z37 strain was isolated from Rattus norvegicus and identified as SEO virus by serological test. The M segment cDNA of Hantavirus Z37 strain was obtained by reverse transcripti on and polymerase chain reaction, subsequently cloned into pGEM-T vector. The s equence of positive recombinants was determined by the method of dideoxy chain t ermination, which revealed that the M genomic segment is 3651 nucleotide in len gth with a predicated long open reading frame encoding a protein of 1133 amino acids. Comparison with HNT type (76-118, A9, HV-114 strains) indicated that th ere were 71.8%～72.1% homology at the nucleotide level, 76.2～76.7% homology at the amino acid level. Comparison with SEO type (R22,L99,80-39 strains) showed 95.3～96.1 homology at the nucleotide level, 95.3～99.2% homology at the amino acid level. The results of nucleotide and amino acid comparison indicated that Z 37 strain is SEO viruses in molecular level.