CAJ | 학술논문

将带有目的基因PSP94-TNFαD11a的pcDNA3.0质粒DNA，以50μg/只剂量注射到39只雄性昆明小鼠的股四头肌内，第2、3、4、5、10、15、20、25天分别摘眼球取血收集血清，每组3只动物血清混合。同时取注射部位骨骼肌，研磨后离心取上清。用ELSIA方法检测血清和骨骼肌上清中融合蛋白的表达，观察不同时间的蛋白表达水平。提取14天骨骼肌组织的总RNA，进行RT-PCR分析目的基因mRNA的表达水平。结果，在裸质粒注射后9天可检出目的蛋白的表达，第14天出现表达高峰，观察至25天仍可检察到蛋白表达。
장대유목적기인PSP94-TNFαD11a적pcDNA3.0질립DNA，이50μg/지제량주사도39지웅성곤명소서적고사두기내，제2、3、4、5、10、15、20、25천분별적안구취혈수집혈청，매조3지동물혈청혼합。동시취주사부위골격기，연마후리심취상청。용ELSIA방법검측혈청화골격기상청중융합단백적표체，관찰불동시간적단백표체수평。제취14천골격기조직적총RNA，진행RT-PCR분석목적기인mRNA적표체수평。결과，재라질립주사후9천가검출목적단백적표체，제14천출현표체고봉，관찰지25천잉가검찰도단백표체。
PSP94-TNFαD11a naked plasmid intramuscular injection on intact mice.PSP94-TNFαD11a cDNA cloned in pcDNA3,an eukarocytic experssion plasmid,was intramuscularly injected into the quadriceps muscle of 39 mice (50μg each). Serum was collected .The expression of fusion protein was detected by ELISA.Uninjected mice were used as control.RNA of PSP94-TNFαD11a from tissue of skeletal muscle was determined using reverse transcription polymerase chain reaction analysis.The result showed that the levels of expressed protein were remarkably elevated in the serum nine days after the injection of naked plasmid DNA ,peaking on the fourteen days ,and maintaining at a detectable level up to 25 days.