CAJ | 학술논문

目的：探讨寻常型银屑病(psoriasis vulgaris,PV) 与人类白细胞抗原(human leukoc yte antigens,HLA)复合体C基因座位第73位氨基酸密码子的相关性。方法：采用聚合酶链反应(Polymerase Chain Reaction,PCR)--变性聚丙烯酰胺凝胶电泳-DNA银染、测序，检测了10 4例PV患者和110例健康对照者的HLA-C基因第73位氨基酸密码子。结果：PV患者编码HLA -C抗原第73位氨基酸的核苷酸，为丙氨酸序列(73alanine sequence,73 Ala) 的频率比健康对照组显著性增高(81.7%比53.6%，RR＝3.78，χ2＝19.21，P＜0.005)；为苏氨酸序列(73threonine sequence, 73Thr)的频率比健康对照组显著性降低(77.9%比89.1%，RR＝0.43，χ2＝4.92，P＜0.05)。4个PCR产物测序结果证实扩增片段确实为所需目的DN A。结论：HLA-C第73位密码子与PV发病率存在相关性；HLA-C的 73 Ala可能是P V的易感因素，而其73Thr似乎是PV的保护性因素，且为银屑病病因及发病机制的探讨提供了一些信息。
목적：탐토심상형은설병(psoriasis vulgaris,PV) 여인류백세포항원(human leukoc yte antigens,HLA)복합체C기인좌위제73위안기산밀마자적상관성。방법：채용취합매련반응(Polymerase Chain Reaction,PCR)--변성취병희선알응효전영-DNA은염、측서，검측료10 4례PV환자화110례건강대조자적HLA-C기인제73위안기산밀마자。결과：PV환자편마HLA -C항원제73위안기산적핵감산，위병안산서렬(73alanine sequence,73 Ala) 적빈솔비건강대조조현저성증고(81.7%비53.6%，RR＝3.78，χ2＝19.21，P＜0.005)；위소안산서렬(73threonine sequence, 73Thr)적빈솔비건강대조조현저성강저(77.9%비89.1%，RR＝0.43，χ2＝4.92，P＜0.05)。4개PCR산물측서결과증실확증편단학실위소수목적DN A。결론：HLA-C제73위밀마자여PV발병솔존재상관성；HLA-C적 73 Ala가능시P V적역감인소，이기73Thr사호시PV적보호성인소，차위은설병병인급발병궤제적탐토제공료일사신식。
To explore the correlation between codons at position 73 of HLA-C and psoriasis vulgaris for people of Han nationality in Hubei Province.Methods:Pol ymerase Chain Reaction (PCR)-denature polyacrylamide gel electrophoresis-DNA s ilver stain technique and DNA sequencing for PCR products were employed to detec t the codons at position 73 of HLA-C in 104 cases with psoriasis vulgaris and 110 healthy controls.Results:The frequency of nucleotide sequence coding alani ne at position 73 of HLA-C antigens(73Ala) in the cases was 81.7%,while t hat in healthy controls was 53.6%.The former was significantly higher than the latte r (RR＝3.87,χ2＝19.12,P＜0.005).But the frequency o f nucleotide sequence coding threonine at position 73 of HLA-C antigens (73 Thr) in PV patients was significantly lower than that in healthy controls (77. 9% versus 89.1%,RR＝0.43,χ2＝4.92,P＜0.05).DNA sequencing for fou r PCR products ascertained that the PCR products were the DNA fragments which we re needed.Conclusions:In PV patients of Han nationality in Hubei Province,73 Ala of HLA-C gene may play a role in susceptibility to PV,but 73　Thr of HLA-C gene seems to be a resistant factor to PV.This study provides some message for exploring etiology and pathogenesis of PV.