CAJ | 학술논문

分别用NDV疫苗株和不同稀释度的B95毒株，经不同免疫途径进行雏鸡的免疫，当雏鸡NDV母源抗体下降到较低水平时，B95病毒液50倍稀释组在免疫后5 d即可见抗体滴度上升，到达高峰时抗体效价比免疫前上升4.6个滴度，在攻毒试验中，除B95 10 000倍稀释组外，其余B95各组在免疫后15 d、30d攻毒保护率均为5/5，即全部保护。当在雏鸡母源抗体较高时免疫，B95病毒液50倍稀释组抗体达高峰时其效价比对照组高2.5个滴度，结果表明，该组与Clone30疫苗组免疫效果相当，能使机体产生良好的免疫。
분별용NDV역묘주화불동희석도적B95독주，경불동면역도경진행추계적면역，당추계NDV모원항체하강도교저수평시，B95병독액50배희석조재면역후5 d즉가견항체적도상승，도체고봉시항체효개비면역전상승4.6개적도，재공독시험중，제B95 10 000배희석조외，기여B95각조재면역후15 d、30d공독보호솔균위5/5，즉전부보호。당재추계모원항체교고시면역，B95병독액50배희석조항체체고봉시기효개비대조조고2.5개적도，결과표명，해조여Clone30역묘조면역효과상당，능사궤체산생량호적면역。
Chicken were immunized with different Newcastle disease virus (NDV)vaccines or with B95 strain in different dilutions or by different immunization methods. When containing a low level of maternal NDV antibody, chicken immunized with B95 strain in a dilution of 50 times could be observed a increasing in HI titre 5 days after immunization, and with a highest level of 4.6 titre higher than control group. In challenge experiment, each B95 group except in a dilution of 10 000 times could be protected completely 15 or 30 days after immunization. ff chicken were immunized at a high maternal level, HI antibody titre of the B95 group in a dilution of 50 times was 2.5 higher than control group, which was similar with Clone30 group.