CAJ | 학술논문

为研究STR位点D2S441的遗传多态性，为法医学应用提供基础数据，应用PCR及PAG电泳技术对260名成都地区汉族无关个体进行了调查，共检出9个等位基因及26种基因型，首次获得汉族群体频率分布,其等位基因片段大小范围为131～155bp。该位点基因型频率分布符合Hardy-Weinberg平衡。家系调查证实了等位基因的传递遵循孟德尔遗传规律。其个人识别能力（Dp）、杂合度（H）、多态性信息含量（PIC）和非父排除率（PE）分别为0.9084、0.7885、0.7390和0.5778，表明该位点在法医学个人识别及亲子鉴定中具有较高的实用价值。
위연구STR위점D2S441적유전다태성，위법의학응용제공기출수거，응용PCR급PAG전영기술대260명성도지구한족무관개체진행료조사，공검출9개등위기인급26충기인형，수차획득한족군체빈솔분포,기등위기인편단대소범위위131～155bp。해위점기인형빈솔분포부합Hardy-Weinberg평형。가계조사증실료등위기인적전체준순맹덕이유전규률。기개인식별능력（Dp）、잡합도（H）、다태성신식함량（PIC）화비부배제솔（PE）분별위0.9084、0.7885、0.7390화0.5778，표명해위점재법의학개인식별급친자감정중구유교고적실용개치。
Using PCR and PAG, followed by silver staining, the tetrameric STR D2S441 locus was studied in 260 unrelated Chinese individuals living in Chengdu. 9 alleles and 26 genotypes were observed. The range of fragment size was 131bp to 155bp. The genotype distribution of D2S441 locus in Han population was in accordance with Hardy-Weinberg equilibrium. Family survey confirmed Mendelian inheritance of alleles. The discriminating power (Dp), observed heterozygosity (H), polymorphism information content (PIC) and power of exclusion (PE) were 0.9084, 0.7885, 0.7390 and 0.5778 respectively. The results demonstrated that this locus was highly polymorphic and could be used for forensic identification and paternity testing.