CAJ | 학술논문

研究了乙酰胺的分光光度分析方法。该方法用溴水氧化酰胺，其氧化产物可把I-氧化成I2，I2再和淀粉-KI生成有色络合物来测定酰胺的含量，主要适用于伯酰胺的分析。方法在5×10-6～1×10-4 g／25 mL范围内符合比耳定律，表观摩尔吸光系数ε576nm=4．5×104L·mol-1·cm-1。该法灵敏、快速、重现性好。
연구료을선알적분광광도분석방법。해방법용추수양화선알，기양화산물가파I-양화성I2，I2재화정분-KI생성유색락합물래측정선알적함량，주요괄용우백선알적분석。방법재5×10-6～1×10-4 g／25 mL범위내부합비이정률，표관마이흡광계수ε576nm=4．5×104L·mol-1·cm-1。해법령민、쾌속、중현성호。
The spectrophotometric determination of aimde was stuided. The method is sensitive, rapid and repreducible. The method is based on the bromine oxidation of the amide. The amide oxidation product oxidizes iodide ion to iodine, which is reacted with potatinium iodide-starch to form a color complex for spectrophotometric determination of amide. The procedure is specific to primary amides. Beer's law is obeyed for amide in the range of 5 × 10-6～1 × 104 g/25 mL at maxinium wavelength 576 nm with apparent εs76nm of 4.5×104 L · mol-1 · cm-1.