西南农业学报
西南農業學報
서남농업학보
SOUTHWEST CHINA JOURNAL OF AGRICULTURAL SCIENCES
2000年
3期
6-11
,共6页
张尧忠%IDA%飞田哲%仙北俊弘%长峰司
張堯忠%IDA%飛田哲%仙北俊弘%長峰司
장요충%IDA%비전철%선북준홍%장봉사
亚洲栽培稻%耐盐性%分类%地理差异%等位基因
亞洲栽培稻%耐鹽性%分類%地理差異%等位基因
아주재배도%내염성%분류%지리차이%등위기인
rice%salt-tolerance%geographical differences%ester ase marker
在水稻发芽期,筛选耐盐性品种的最适NaCl浓度是1.5% 。在这个浓度下,调查了九个亚洲国家的306个品种的耐盐性。参照程氏形态分类法的稃毛标准将参试品种分成籼和粳,籼稻品种比粳稻更耐盐。酯酶(Est)同工酶分析也印证了这一结果。酯酶同工酶的等位基因Est-22、Est-32和Est-41可以用来鉴别籼稻品种,而Est-2°、Est-31和Est-4°可以识别粳稻品种。具有等位基因Est-22、Est -33和Est-41的品种比具有Est-2°、Est-31和Est-4°的品种更耐盐。比较七个酯酶同工酶酶谱的品种耐盐性,发现有70%的具有Est-22的品种在1.5%NaCl浓度下发芽率超过50%。因此,等位基因Est-22可能是品种耐盐性的一个标志。大多数马来西亚的品种是耐盐的,其次为缅甸、中国和孟加拉的品种。大多数泰国、菲律宾和日本的品种对盐敏感
在水稻髮芽期,篩選耐鹽性品種的最適NaCl濃度是1.5% 。在這箇濃度下,調查瞭九箇亞洲國傢的306箇品種的耐鹽性。參照程氏形態分類法的稃毛標準將參試品種分成秈和粳,秈稻品種比粳稻更耐鹽。酯酶(Est)同工酶分析也印證瞭這一結果。酯酶同工酶的等位基因Est-22、Est-32和Est-41可以用來鑒彆秈稻品種,而Est-2°、Est-31和Est-4°可以識彆粳稻品種。具有等位基因Est-22、Est -33和Est-41的品種比具有Est-2°、Est-31和Est-4°的品種更耐鹽。比較七箇酯酶同工酶酶譜的品種耐鹽性,髮現有70%的具有Est-22的品種在1.5%NaCl濃度下髮芽率超過50%。因此,等位基因Est-22可能是品種耐鹽性的一箇標誌。大多數馬來西亞的品種是耐鹽的,其次為緬甸、中國和孟加拉的品種。大多數泰國、菲律賓和日本的品種對鹽敏感
재수도발아기,사선내염성품충적최괄NaCl농도시1.5% 。재저개농도하,조사료구개아주국가적306개품충적내염성。삼조정씨형태분류법적부모표준장삼시품충분성선화갱,선도품충비갱도경내염。지매(Est)동공매분석야인증료저일결과。지매동공매적등위기인Est-22、Est-32화Est-41가이용래감별선도품충,이Est-2°、Est-31화Est-4°가이식별갱도품충。구유등위기인Est-22、Est -33화Est-41적품충비구유Est-2°、Est-31화Est-4°적품충경내염。비교칠개지매동공매매보적품충내염성,발현유70%적구유Est-22적품충재1.5%NaCl농도하발아솔초과50%。인차,등위기인Est-22가능시품충내염성적일개표지。대다수마래서아적품충시내염적,기차위면전、중국화맹가랍적품충。대다수태국、비률빈화일본적품충대염민감
It was concluded that the optimum NaCl concentration was 1.5% for screening salt-tolerant varieties in the germination stage.At thi s concentration, the germination rates of 275 varieties from nine Asian countrie s were investigated.Among the six morphological characters used by Cheng (1988), glume hairinss was the most reliable in Indica-Japonica classification. Indica varieties were more salt-tolerant than Japonica ones. This result corresponded to esterase isozyme analysis.Alleles Est-22,Est-32 and Est-41 were us ed in identifying Indica varieties. Alleles Est-20,Est-31 and Est-40 served as identifying Japonica varieties. Varieties carrying alleles Est-22, Est-32and Est-41 were more salt-tolerant than those carrying Est-2 0,Est-31 and Est-40. According to salt-tolerant comparison among the s even esterase genotypes,the three alleles of locus Est-2 showed quite different associations with respect to the degree of salt tolerance. Seventy percent of t he varieties with alleles Est-22 had more than 50% of germination rate at 1 .5% NaCl.Allele Est-22 seemed to be a marker of rice salt tolerance.Most of the varieties from Malaysia were salt-tolerant,followed by varieties from Thai land,the Philippines and Japan were salt-susceptible.