CAJ | 학술논문

生物膜在自然界无处不在,但生物膜造成的腐蚀却基本上被忽视.本文展示了几种化学和微生物学新方法在海水微生物腐蚀研究中的应用.原子力显微镜用来揭示生物最初形成的机理和钢在受污染海水中的腐蚀程度,16 Sr DNA/RNA技术则用来分析生物膜中的微生物组成.试验结果表明,微生物腐蚀在6 d内就已经开始了,腐蚀体积与时间的2.83次方成正比;腐蚀生物膜中的微生物以硫酸还原菌(脱硫弧菌科)为最多,其次是梭状芽孢杆菌.
생물막재자연계무처불재,단생물막조성적부식각기본상피홀시.본문전시료궤충화학화미생물학신방법재해수미생물부식연구중적응용.원자력현미경용래게시생물최초형성적궤리화강재수오염해수중적부식정도,16 Sr DNA/RNA기술칙용래분석생물막중적미생물조성.시험결과표명,미생물부식재6 d내취이경개시료,부식체적여시간적2.83차방성정비;부식생물막중적미생물이류산환원균(탈류호균과)위최다,기차시사상아포간균.
Biofilm is ubiquitous in nature. However, corrosin caused by biofilm is still by and large overlooked. This presentation is to demonstrate the applications of several newly developed analytical techniques in chemisrty and microbiology for the study of marine biocorrosion on steel. AFM(atomic force microscopy) was applied to investigate the initial formation mechanism of biofilm and the degree of corrosion of steel in polluted seawater. DNA/RNA-related molecular techniques were used to analyze the microbial composition corrosive biofilm. Results showed that microbial corrosion began within six days, and the corroded volume in the increased as a power function of time with an index 2.83. Most of the microbes identified in the corrosive biofilm were sulfate-reducing Desul fovibrionaceae(46. 5%), followed by Clostridiaceae(29.4%).