CAJ | 학술논문

目的确认肝再生过程中参与调控肝细胞有丝分裂的EGR1靶向基因CDC20, 并对其作用进行评价.方法通过cDNA微阵列分析,比较切肝后48 h野生鼠和EGR1基因敲出鼠的肝组织基因表达.使用RT-PCR法测定再生肝组织中野生鼠和EGR1基因敲出鼠的CDC20基因表达谱,并将其切肝后48h的结果与同一时间点的EGR1蛋白质表达水平进行比较.结果切肝后前12h肝组织CDC20基因的表达水平无变化,但于36h开始上升并于48h达到顶峰,这个表达曲线恰好与肝再生过程中EGR1蛋白质的表达特点一致.此外,与EGR1基因敲出鼠相比,野生鼠切肝后48h的CDC20基因表达水平明显升高(P<0.02).结论 EGR1基因敲出鼠的肝细胞有丝分裂进程受损与肝再生过程中CDC20基因表达水平降低有关;提示:受EGR1调控的CDC20基因的表达在肝再生过程中肝细胞有丝分裂进程中起重要作用.
목적학인간재생과정중삼여조공간세포유사분렬적EGR1파향기인CDC20, 병대기작용진행평개.방법통과cDNA미진렬분석,비교절간후48 h야생서화EGR1기인고출서적간조직기인표체.사용RT-PCR법측정재생간조직중야생서화EGR1기인고출서적CDC20기인표체보,병장기절간후48h적결과여동일시간점적EGR1단백질표체수평진행비교.결과절간후전12h간조직CDC20기인적표체수평무변화,단우36h개시상승병우48h체도정봉,저개표체곡선흡호여간재생과정중EGR1단백질적표체특점일치.차외,여EGR1기인고출서상비,야생서절간후48h적CDC20기인표체수평명현승고(P<0.02).결론 EGR1기인고출서적간세포유사분렬진정수손여간재생과정중CDC20기인표체수평강저유관;제시:수EGR1조공적CDC20기인적표체재간재생과정중간세포유사분렬진정중기중요작용.
Objective:To identify and evaluate the genetic target of EGR-1,CDC20 gene,which involved in regulating hepatocellular mitotic progression during liver regeneration.Methods:Hepatic gene expressions in wildtype and EGR-1 null mouse liver at 48 hours after partial hepatectomy were compared by cDNA microarray.Pattern of CDC20 gene expression were measured by real time RT-PCR in wildtype and EGR-1 null regenerating mouse liver,and the results at 48 hours after partial hepatectomy were compared with our previous EGR-1 protein expression level at this time point.Results:Hepatic CDC20 gene expression was unchanged over the first 12 hours following partial hepatectomy,and then subsequently increased beginning at 36 hours and peaking at 48 hours after partial hepatectomy,which corresponds closely with that of EGR-1 protein expression during liver regeneration.CDC20 was identified as a more highly expressed gene at 48 hours after partial hepatectomy in wildtype regenerating liver compared with EGR-1 null mouse (P<0.02).Conclusion:Impaired mitotic progression is associated with decreased CDC20 expression in regenerating EGR-1 null mouse liver and it suggests that EGR-1 regulation of CDC20 gene expression is an important determinant of hepatocellular mitotic progression during liver regeneration.