CAJ | 학술논문

背景:造血微环境的异常是造血功能障碍的重要影响因素,但关于造血基质细胞是否对辐射具有敏感性,尚无统一定论.目的:观察放射损伤对小鼠早期骨髓造血基质细胞周期及NDA含量的影响,为深化放射辐射条件下导致的造血功能障碍的认识,并为早期干预环境中的有害因子提供科学依据.设计:以实验动物为研究对象,完全随机分组设计,随机对照实验.单位:一所军医大学高原军事医学系中心实验室、预防医学系防原医学教研室.材料:本实验于2002-10/2003-04在解放军第三军医大学动物实验中心完成.实验昆明种健康雄性小白鼠60只.按随机数字法将小鼠分为放射损伤组和正常对照组各30只.方法:对放射损伤组30只小鼠,用60Co-γ射线照射,距离4 m,照射率1.27 Gy/min.正常对照组30只小鼠未实施干预措施.取放射损伤后3,7 d小鼠骨髓细胞进行体外培养,分别对培养14,21 d的骨髓基质细胞进行检测.主要观察指标:射线照射后骨髓基质细胞集落数量、细胞周期和DNA含量的变化.结果:5.0 Gy照射后,骨髓基质细胞仍能贴壁生长,但骨髓基质细胞集落数量显著低于正常(P<0.01).照射7 d后的骨髓基质细胞集落数量虽比照射后3 d所增加,但恢复缓慢,延长培养时间,显示照射鼠的骨髓基质细胞增殖受抑.流式细胞仪检测结果表明放射损伤组照射3,7 d后G2+M期细胞(2.60±0.41,4.20±1.27)和DNA含量(58 40±0.79.61.17±1.35)显著低于正常对照组(12.60±0.75,78.57±0.83)(P<0.05～0.01).结论:骨髓造血基质细胞具有较高的辐射敏感性,且持续时间较久.
배경:조혈미배경적이상시조혈공능장애적중요영향인소,단관우조혈기질세포시부대복사구유민감성,상무통일정론.목적:관찰방사손상대소서조기골수조혈기질세포주기급NDA함량적영향,위심화방사복사조건하도치적조혈공능장애적인식,병위조기간예배경중적유해인자제공과학의거.설계:이실험동물위연구대상,완전수궤분조설계,수궤대조실험.단위:일소군의대학고원군사의학계중심실험실、예방의학계방원의학교연실.재료:본실험우2002-10/2003-04재해방군제삼군의대학동물실험중심완성.실험곤명충건강웅성소백서60지.안수궤수자법장소서분위방사손상조화정상대조조각30지.방법:대방사손상조30지소서,용60Co-γ사선조사,거리4 m,조사솔1.27 Gy/min.정상대조조30지소서미실시간예조시.취방사손상후3,7 d소서골수세포진행체외배양,분별대배양14,21 d적골수기질세포진행검측.주요관찰지표:사선조사후골수기질세포집락수량、세포주기화DNA함량적변화.결과:5.0 Gy조사후,골수기질세포잉능첩벽생장,단골수기질세포집락수량현저저우정상(P<0.01).조사7 d후적골수기질세포집락수량수비조사후3 d소증가,단회복완만,연장배양시간,현시조사서적골수기질세포증식수억.류식세포의검측결과표명방사손상조조사3,7 d후G2+M기세포(2.60±0.41,4.20±1.27)화DNA함량(58 40±0.79.61.17±1.35)현저저우정상대조조(12.60±0.75,78.57±0.83)(P<0.05～0.01).결론:골수조혈기질세포구유교고적복사민감성,차지속시간교구.
BACKGROUND: Abnormal hematopoietic microenvironment is an important factor causing dyshematopoiesis. However, no consensus has been reached on the sensitivity of hematopoietic stromal cells to irradiation.OBJECTIVE: To observe the changes of marrow stromal cells (MSCs) cycle and DNA content during the early stage of irradiation damage in mice, so as to further understand dyshematopoiesis due to radiation and provide scientific basis to avoid deleterious factors in hematopoietic environment.DESIGN: Completely randomized grouping and randomized controlled study based on the experimental animals.SETTING: Central laboratory of altitude military affairs medical department and altitude research institute of preventive medicine department, a military medical university of Chinese PLA.MATERIALS: This study was carried out at the Experimental Animal Center of Third Military Medical University between October 2002 and April 2003. A total of 60 healthy male Kunming mice were randomly divided into irradiation damage group and healthy control group, each having 30 mice.METHODS: The 30 mice in irradiation damage group were exposed to 60Co-γ of irradiation at a dose rate of 1.27 Gy/minutes within a distance of 4 m. Then the mice' marrow cells were harvested at day 3 and day 7 after irradiation, and were cultured in vitro for 14 days and 21 days for observation. Meanwhile the other 30 healthy mice unexposed to irradiation were considered as normal controls.MAIN OUTCOME MEASURES: Post-radiation number of MSCs colonies,cell cycle and DNA content.RESULTS: Although MSCs could grow and be adhered to walls after being exposed to irradiation of 5.0 Gy/s, the number of MSCs colonies was found significantly decreased compared to that of rnormal control group( P < 0.01 ).The colony number of the MSCs irradiated for 7 days obviously increased than that of MSCs irradiated for 3 days; however, MSCs recovered slowly and resulted in prolonged culture time, indicating the inhibited proliferation of MSCs due to irradiation damage. Results of flow cytometry showed that cells in G2+ M phase(2.60±0.41, 4.20±1.27) and DNA content (58.40±0.79,61.17 ± 1.35) in irradiation groups after 3-day and 7-day irradiation were obviously lower than those of normal control group(12.60 ±0. 75, 78.57±0. 83)(P <0.05-0.01).CONCLUSION: MSCs have relatively high sensitivity to irradiation damage and longer persisting period.