CAJ | 학술논문

目的:开发一种快速检测血清中日本血吸虫抗体的免疫诊断方法.方法:用胶体碳标记日本血吸虫成熟虫卵可溶性抗原(SEA),与待测血清中日本血吸虫抗体结合,再用SEA包被在硝酸纤维素膜(NC膜)上以捕捉碳标SEA-血吸虫抗体复合物,达到检测血吸虫抗体的目的. 结果:检测137份血清,与间接血凝法(indirect haemagglutination assay,IHA)检测结果一致性为98.54%,如经IHA试剂盒检测呈阳性者即视为阳性血清,则该方法的检测敏感性为98.99%,特异性为97.37%;且检测条带的灰度与血清效价具有曲线关系,因而可以对待测血清作半定量分析. 结论:胶体碳试条层析法操作简便,快速,具有较高的敏感性和特异性,适合作血吸虫现场检测.
목적:개발일충쾌속검측혈청중일본혈흡충항체적면역진단방법.방법:용효체탄표기일본혈흡충성숙충란가용성항원(SEA),여대측혈청중일본혈흡충항체결합,재용SEA포피재초산섬유소막(NC막)상이포착탄표SEA-혈흡충항체복합물,체도검측혈흡충항체적목적. 결과:검측137빈혈청,여간접혈응법(indirect haemagglutination assay,IHA)검측결과일치성위98.54%,여경IHA시제합검측정양성자즉시위양성혈청,칙해방법적검측민감성위98.99%,특이성위97.37%;차검측조대적회도여혈청효개구유곡선관계,인이가이대대측혈청작반정량분석. 결론:효체탄시조층석법조작간편,쾌속,구유교고적민감성화특이성,괄합작혈흡충현장검측.
Objective To develop a rapid and simple immunoassay to detect antibodies in the sera of patients infect Schistosoma Japonicum (S.Japonicum). Methods Soluble egg antigen (SEA) of S.Japonicum conjugated with colloidal carbon in advance was used to react with the antibodies in the sera of patients with Schistosomiasis. Then the carbon-antigen-antibody complex would be captured by SEA which had been absorbed on the nitrocellulose membrane and a gray band was shown. Results A total of 137 sera samples from S.Japonicum epidemic area were tested, and the consistency, sensitivity, and specificity of colloidal carbon dipstick assay were 98.54%, 98.99%, and 97.37%, respectively, compared with the IHA method. The gray scale of bands on the dipstick was curvilinear to serum titer which revealed that the assay could be used semi-quantitatively in serum analysis.Conclusion Colloidal carbon dipstick assay is not only rapid and simple, but also sensitive and specific for the detection of serum antibodies of Schistosomiasis Japonica. It will be a practical immunological assay for the diagnosis of Schistosomiasis in the field testing.