氨基酸和生物资源
氨基痠和生物資源
안기산화생물자원
AMINO ACIDS AND BIOTIC RESOURCES
2010年
1期
31-33
,共3页
任艳%万德光%任小丹%张志丹%国锦琳
任豔%萬德光%任小丹%張誌丹%國錦琳
임염%만덕광%임소단%장지단%국금림
猪血%多肽%酶解%电泳(SDS-PAGE)
豬血%多肽%酶解%電泳(SDS-PAGE)
저혈%다태%매해%전영(SDS-PAGE)
porcine blood%peptide%enzymolysis%electrophoresis (SDS-PAGE)
通过体外模拟体内条件,选取主要消化酶胃蛋白酶、胰蛋白酶对猪血脱色液进行酶解,以高效酶木瓜蛋白酶和1394酶作为对照,结合酶解后多肽总含量以及＜3 kDa的多肽得率进行酶解效果的综合评价,得到胃蛋白酶为最佳酶解酶.
通過體外模擬體內條件,選取主要消化酶胃蛋白酶、胰蛋白酶對豬血脫色液進行酶解,以高效酶木瓜蛋白酶和1394酶作為對照,結閤酶解後多肽總含量以及＜3 kDa的多肽得率進行酶解效果的綜閤評價,得到胃蛋白酶為最佳酶解酶.
통과체외모의체내조건,선취주요소화매위단백매、이단백매대저혈탈색액진행매해,이고효매목과단백매화1394매작위대조,결합매해후다태총함량이급＜3 kDa적다태득솔진행매해효과적종합평개,득도위단백매위최가매해매.
Through imitating body environment in vitro, the main digestive enzymes pepsin and trypsin were selected to hydrolyze the decolorized porcine blood. 1394 enzyme and papain enzyme were taken as the control. Based on the content of <3 kDa peptides, pepsin was evaluated as the optimum enzyme.
