中国中西医结合杂志
中國中西醫結閤雜誌
중국중서의결합잡지
CHINESE JOURNAL OF INTEGRATED TRADITIONAL AND WESTERN MEDICINE
2010年
1期
68-71
,共4页
赵永华%刘煜德%刘乃维%李焕仪%项平
趙永華%劉煜德%劉迺維%李煥儀%項平
조영화%류욱덕%류내유%리환의%항평
颐年降压饮%自发性高血压%内皮细胞增殖%过氧化物酶体增殖物激活受体γ
頤年降壓飲%自髮性高血壓%內皮細胞增殖%過氧化物酶體增殖物激活受體γ
이년강압음%자발성고혈압%내피세포증식%과양화물매체증식물격활수체γ
Yinian Jiangya Decoction%spontaneous hypertension%endothelial cell proliferation%peroxisome proliferator-activated receptor-γ
目的 观察颐年降压饮含药血清对体外原代培养的自发性高血压大鼠(spontaneously hyperten-sive rats,SHR)内皮细胞增殖及PPAR-γmRNA表达的影响.方法 取SHR主动脉内皮细胞进行原代培养,取3代后内皮细胞用于实验,SD大鼠40只随机分为正常血清对照组和颐年降压饮高、中、低剂量组,给与高脂饮食喂养,分别灌服生理盐水和高、中、低剂量颐年降压饮(分别含生药5.2 g/mL、2.6 g/mL、1.3 g/mL),给药20天麻醉后开始收集血清,经灭活后作用各组内皮细胞.MTT法检测不同浓度血清作用各组2、4、8、16、24、48 h细胞活性;RT-PCR法检测作用4、8、16、24 h内皮细胞PPAR-γmRNA表达情况.结果 MTT检测OD值发现,4、8 h颐年降压饮高、中、低剂量组OD值均高于正常血清对照组,差异有统计学意义(P<0.05),而颐年降压饮3剂量组间差异无统计学意义(P>0.05);16 h颐年降压饮中剂量组OD值上升低于其他组,差异有统计学意义(P<0.05);24 h各组OD值均下降,但颐年降压饮高、中剂量组下降比低剂量、正常血清对照组明显,差异有统计学意义(P<0.05);48 h各组OD值继续下降,颐年降压饮高剂量组比正常血清对照组下降更明显(P<0.05).RT-PCR检测不同血清作用内皮细胞4 h时,各组PPAR-γmRNA表达差异无统计学意义(P>0.05);8 h时颐年降压饮高剂量组PPARγmRNA表达明显低于其他各组(P<0.05);16 h颐年降压饮各剂量组PPAR-γmRNA表达比正常血清对照组高(P<0.05),其中颐年降压饮高剂量组PPARymRNA表达最高,与其他组比较,差异有统计学意义(P<0.01);24 h各组PPAR-γmRNA表达有所下降,但颐年降压饮低剂量组PPAR-γmRNA表达仍高于正常血清对照组(P<0.05).结论 颐年降压饮对内皮细胞增殖呈双向调节作用,或许与调节PPAR-γmRNA表达有关.颐年降压饮所具有的上调及维持PPAR-γmRNA表达作用,可能是该方药具有保护血管内皮功能,降低血压的机制之一.
目的 觀察頤年降壓飲含藥血清對體外原代培養的自髮性高血壓大鼠(spontaneously hyperten-sive rats,SHR)內皮細胞增殖及PPAR-γmRNA錶達的影響.方法 取SHR主動脈內皮細胞進行原代培養,取3代後內皮細胞用于實驗,SD大鼠40隻隨機分為正常血清對照組和頤年降壓飲高、中、低劑量組,給與高脂飲食餵養,分彆灌服生理鹽水和高、中、低劑量頤年降壓飲(分彆含生藥5.2 g/mL、2.6 g/mL、1.3 g/mL),給藥20天痳醉後開始收集血清,經滅活後作用各組內皮細胞.MTT法檢測不同濃度血清作用各組2、4、8、16、24、48 h細胞活性;RT-PCR法檢測作用4、8、16、24 h內皮細胞PPAR-γmRNA錶達情況.結果 MTT檢測OD值髮現,4、8 h頤年降壓飲高、中、低劑量組OD值均高于正常血清對照組,差異有統計學意義(P<0.05),而頤年降壓飲3劑量組間差異無統計學意義(P>0.05);16 h頤年降壓飲中劑量組OD值上升低于其他組,差異有統計學意義(P<0.05);24 h各組OD值均下降,但頤年降壓飲高、中劑量組下降比低劑量、正常血清對照組明顯,差異有統計學意義(P<0.05);48 h各組OD值繼續下降,頤年降壓飲高劑量組比正常血清對照組下降更明顯(P<0.05).RT-PCR檢測不同血清作用內皮細胞4 h時,各組PPAR-γmRNA錶達差異無統計學意義(P>0.05);8 h時頤年降壓飲高劑量組PPARγmRNA錶達明顯低于其他各組(P<0.05);16 h頤年降壓飲各劑量組PPAR-γmRNA錶達比正常血清對照組高(P<0.05),其中頤年降壓飲高劑量組PPARymRNA錶達最高,與其他組比較,差異有統計學意義(P<0.01);24 h各組PPAR-γmRNA錶達有所下降,但頤年降壓飲低劑量組PPAR-γmRNA錶達仍高于正常血清對照組(P<0.05).結論 頤年降壓飲對內皮細胞增殖呈雙嚮調節作用,或許與調節PPAR-γmRNA錶達有關.頤年降壓飲所具有的上調及維持PPAR-γmRNA錶達作用,可能是該方藥具有保護血管內皮功能,降低血壓的機製之一.
목적 관찰이년강압음함약혈청대체외원대배양적자발성고혈압대서(spontaneously hyperten-sive rats,SHR)내피세포증식급PPAR-γmRNA표체적영향.방법 취SHR주동맥내피세포진행원대배양,취3대후내피세포용우실험,SD대서40지수궤분위정상혈청대조조화이년강압음고、중、저제량조,급여고지음식위양,분별관복생리염수화고、중、저제량이년강압음(분별함생약5.2 g/mL、2.6 g/mL、1.3 g/mL),급약20천마취후개시수집혈청,경멸활후작용각조내피세포.MTT법검측불동농도혈청작용각조2、4、8、16、24、48 h세포활성;RT-PCR법검측작용4、8、16、24 h내피세포PPAR-γmRNA표체정황.결과 MTT검측OD치발현,4、8 h이년강압음고、중、저제량조OD치균고우정상혈청대조조,차이유통계학의의(P<0.05),이이년강압음3제량조간차이무통계학의의(P>0.05);16 h이년강압음중제량조OD치상승저우기타조,차이유통계학의의(P<0.05);24 h각조OD치균하강,단이년강압음고、중제량조하강비저제량、정상혈청대조조명현,차이유통계학의의(P<0.05);48 h각조OD치계속하강,이년강압음고제량조비정상혈청대조조하강경명현(P<0.05).RT-PCR검측불동혈청작용내피세포4 h시,각조PPAR-γmRNA표체차이무통계학의의(P>0.05);8 h시이년강압음고제량조PPARγmRNA표체명현저우기타각조(P<0.05);16 h이년강압음각제량조PPAR-γmRNA표체비정상혈청대조조고(P<0.05),기중이년강압음고제량조PPARymRNA표체최고,여기타조비교,차이유통계학의의(P<0.01);24 h각조PPAR-γmRNA표체유소하강,단이년강압음저제량조PPAR-γmRNA표체잉고우정상혈청대조조(P<0.05).결론 이년강압음대내피세포증식정쌍향조절작용,혹허여조절PPAR-γmRNA표체유관.이년강압음소구유적상조급유지PPAR-γmRNA표체작용,가능시해방약구유보호혈관내피공능,강저혈압적궤제지일.
Objective To observe the effects of Yinian Jiangya (YNJY) Decoction contained serum on cell proliferation of primary cultured endothelial cells of spontaneously hypertensive rat (SHR) and the peroxisome proliferator-activated receptor-γ (PPAR-γ) mRNA expression in the cells. Methods SD rats were fed with high-lipid diet and different doses (high, medium and low, containing crude drug of 5.2 g/mL, 2.6 g/mL, 1.3 g/mL respectively) of YNJY respectively and the serum contained different doses of YNJY (S-YNJY) was prepared by collecting the rats' serum after 20 days of feeding. The serum obtained from SD rats fed with normal diet, I.e. Drug free serum (S-free) was taken for control. Primary cultured endothelial cells of SHR were treated respectively with S-YNJY in different doses (treated groups) and S-free (control group), the cell activity and the mRNA expression of PPARγ in cells of all groups were detected by MTT and RT-PCR respectively at different time points: 2, 4, 8, 16 and 24 h after treatment. Results MTT test showed that at time points of 4 and 8 h, the OD value raised in the treated groups with insignificant difference among them (P>0.05), but was higher than that in the control group (P<0.05);at 16 h, it increased but showed a smaller increment in the medium-dose treated group (P<0.05); at 24 h, it decreased in all groups, but the decrement in the high-and medium-dose treated groups was more remarkable (P<0.05); at 48 h, it decreased continuously, with the decrement more significant in the high-dose group than in the control group (P<0.05). RT-PCR detection showed that at 4 h, the expression of PPARγ mRNA was not changed significantly in all groups (P>0.05); at 8 h, it was remarkably lower in high-dose group than in others (P<0.05); at 16 h, it was higher in the three treated groups than in the control group (P<0.05), with a highest level shown in the high-dose treated group (P<0.01); at 24 h, the expression decreased in all groups, but the level in the low-dose treated group was still higher than that in the control group (P<0.05). Conclusions YNJY Decoction shows a two-way regulation on endothelial cells proliferation, and which is negatively dose-dependent in the late stage. The regulation is perhaps relevant to the regulating of the PPARγ mRNA expressions. The PPARγ mRNA expression up-regulating and maintaining effects of YNJY Decoction may be one of the mechanisms for its vascular endothelial cell protection and blood pressure suppressing.
