蚕业科学
蠶業科學
잠업과학
ACTA SERICOLOGICA SINICA
2010年
2期
323-329
,共7页
吴凡%刘训理%张楠%张莎莎%国辉%张本峰%仇念全
吳凡%劉訓理%張楠%張莎莎%國輝%張本峰%仇唸全
오범%류훈리%장남%장사사%국휘%장본봉%구념전
桑树%硅酸盐细菌%rep-PCR%DNA指纹分析%解钾能力%分类%菌落形态%16S%rDNA
桑樹%硅痠鹽細菌%rep-PCR%DNA指紋分析%解鉀能力%分類%菌落形態%16S%rDNA
상수%규산염세균%rep-PCR%DNA지문분석%해갑능력%분류%균락형태%16S%rDNA
Morus L.%Silicate bacterium%rep-PCR DNA fingerprinting%Potassium-realesing activity%Classification%Co-lonial morphology%16SrDNA
利用选择性培养基,从桑树根际土样中分离获得29个具有解钾能力的细菌分离株,经rep-PCR DNA指纹分析得到24株硅酸盐细菌.通过解钾能力测定,筛选出FK2、FK3、FK8、FK11、FK4′、FK23和PK18 7个具有较强解钾能力的菌株,其中FK2菌株的解钾能力最强,钾增长41.79%.对这7株具有较强解钾能力的细菌进行菌落形态特征观察及16S rDNA序列测定和同源性分析:FK2菌株为假单胞菌属(Pseudomonas sp.),FK3和FK23菌株为中华根瘤菌属(Sinorhizobium sp.),FK11和FK8菌株为根瘤菌属(Rhizobium sp.),FK4′菌株为中慢生根瘤菌属(Mesorhizobium sp.),PK18菌株为屈挠杆菌属(Flexibacter sp.).
利用選擇性培養基,從桑樹根際土樣中分離穫得29箇具有解鉀能力的細菌分離株,經rep-PCR DNA指紋分析得到24株硅痠鹽細菌.通過解鉀能力測定,篩選齣FK2、FK3、FK8、FK11、FK4′、FK23和PK18 7箇具有較彊解鉀能力的菌株,其中FK2菌株的解鉀能力最彊,鉀增長41.79%.對這7株具有較彊解鉀能力的細菌進行菌落形態特徵觀察及16S rDNA序列測定和同源性分析:FK2菌株為假單胞菌屬(Pseudomonas sp.),FK3和FK23菌株為中華根瘤菌屬(Sinorhizobium sp.),FK11和FK8菌株為根瘤菌屬(Rhizobium sp.),FK4′菌株為中慢生根瘤菌屬(Mesorhizobium sp.),PK18菌株為屈撓桿菌屬(Flexibacter sp.).
이용선택성배양기,종상수근제토양중분리획득29개구유해갑능력적세균분리주,경rep-PCR DNA지문분석득도24주규산염세균.통과해갑능력측정,사선출FK2、FK3、FK8、FK11、FK4′、FK23화PK18 7개구유교강해갑능력적균주,기중FK2균주적해갑능력최강,갑증장41.79%.대저7주구유교강해갑능력적세균진행균락형태특정관찰급16S rDNA서렬측정화동원성분석:FK2균주위가단포균속(Pseudomonas sp.),FK3화FK23균주위중화근류균속(Sinorhizobium sp.),FK11화FK8균주위근류균속(Rhizobium sp.),FK4′균주위중만생근류균속(Mesorhizobium sp.),PK18균주위굴뇨간균속(Flexibacter sp.).
29 bacterial isolates that had potassium-releasing activities were obtained frOm mulberry rhizospheric soil through cultivation with selective media.Analysis of repetitive-element PCR(rep-PCR)genomic DNA fingerprinting iden-tiffed 24 silicate bacteria from them.Determination of their potassium-releasing activities indicated that 7 isolates,i.e.FK2,FK3,FK8,FK11,FK4',FK23 and PK18,had high potassium-releasing activities,among which FK2 had the high- est potassium-releasing activity.Its available potassium increment reached 41.79%.Observations on morphological fea-tures of bacterial colonies and phylogenetic analysis of 16SrDNA sequences to the 7 isolates with high potassium-relea-sing activity revealed that FK2 belonged to Pseudomonas sp.,FK3 and FK23 belonged to Sinorhizobium sp.,FK11 and FK8 belonged to Rhizobium sp.,FK4'belonged to Mesorhizobium sp.,and PKl8 belonged to Flexibacter sp.
