CAJ | 학술논문

目的观察咀嚼压力增强对大鼠剩余牙槽嵴中MMP-13表达的变化.方法建立大鼠剩余牙槽嵴创伤性吸收的动物实验模型,于3d、7d、14d、21d、56d后取材,采用SP免疫组织化学方法进行染色,观察MMP-13表达的变化.结果实验组牙槽骨吸收,MMP-13呈强阳性表达,且这种变化随着观察时间的变化而变化,在7d、14d时最明显,与对照组比较有统计学意义(P<0.05);而在28 d、56 d时二者差别不大.结论咀嚼压力增强时,剩余牙槽嵴中MMP-13表达的量增多,且其含量的变化与压力大小有关,力值越大,表达越强.
목적 관찰저작압력증강대대서잉여아조척중MMP-13표체적변화.방법 건립대서잉여아조척창상성흡수적동물실험모형,우3d、7d、14d、21d、56d후취재,채용SP면역조직화학방법진행염색,관찰MMP-13표체적변화.결과 실험조아조골흡수,MMP-13정강양성표체,차저충변화수착관찰시간적변화이변화,재7d、14d시최명현,여대조조비교유통계학의의(P<0.05);이재28 d、56 d시이자차별불대.결론저작압력증강시,잉여아조척중MMP-13표체적량증다,차기함량적변화여압력대소유관,력치월대,표체월강.
Objective To explore the effect of increased masticatory pressure on the expression of MMP-13 in rat residual ridge. Methods A rat model of traumatic absorption of residual ridge was established. The expression of MMP-13 in the tissue samples of residual ridge was detected by SP immuno histochemical assay on days 3,7, 14, 21, and 56. Results As compared with the control group, alveolar bone resorption occurred markedly and MMP-13 was highly expressed in the experimental group, especially on days 7 and 14 (P<0.05); but these changes did not differ significantly on days 28 and 56. Conclusions The expression level of MMP-13 in rat residual ridge is elevated with the increased masticatory pressure. The change in the expression level is associated with the pressure level; the greater the masticatory pressure is, the higher the expression will be.