国际生物制品学杂志
國際生物製品學雜誌
국제생물제품학잡지
INTERNATIONAL JOURNAL OF BIOLOGICALS
2012年
2期
64-66
,共3页
任鹏%姚娟%金艳%汪晓婷%葛俊%李法锦%赵蕾%吉阳%江龙凤%王明丽
任鵬%姚娟%金豔%汪曉婷%葛俊%李法錦%趙蕾%吉暘%江龍鳳%王明麗
임붕%요연%금염%왕효정%갈준%리법금%조뢰%길양%강룡봉%왕명려
巨细胞病毒%疱疹病毒1型,人%酶联免疫吸附测定%婴儿,新生
巨細胞病毒%皰疹病毒1型,人%酶聯免疫吸附測定%嬰兒,新生
거세포병독%포진병독1형,인%매련면역흡부측정%영인,신생
Cytomegalovirus%Herpesvirus 1,human%Enzyme-linked immunosorbent assay%Infant,newborn
目的 了解新生儿单纯疱疹病毒1型(herpes simplex virus type 1,HSV-1)和人巨细胞病毒( human cytomegalovirus,HCMV)血清抗体阳性情况及其与早产儿临床表现的关系.方法 收集新生儿血清共178份(其中健康产妇顺产新生儿脐带血血清114份,早产儿外周血血清64份),分别用HSV-I和HCMV IgG、IgM ELISA试剂盒进行检测.采用x2检验对结果进行比较.结果 178名新生儿中,血清HSV-1 IgG阳性率为83.70％,IgM为阴性；HCMV IgG和IgM阳性率分别为94.38％和0.56％.在114名顺产新生儿中,HSV-1 IgG和IgM阳性率分别为78.07％和0.00％,HCMVIgG和IgM阳性率分别为94.74％和0.88％,HSV-1 IgG和HCMVIgG共阳性率为73.68％.在64名早产儿中,HSV-1 IgG和IgM阳性率分别为93.75％和0.00％,HCMV IgG和IgM阳性率分别为93.75％和0.00％,HSV-1 IgG和HCMV IgG共阳性率为87.50％.早产儿与顺产儿的HCMV IgG阳性率差异无统计学意义(x2=0.07,P＞0.05)；但早产儿HSV-1 IgG阳性率以及HSV-1 IgG和HCMV IgG共阳性率均高于顺产儿,且差异有统计学意义(x2值分别为7.38和4.65,P值均＜0.05).HSV-1 IgG和HCMVIgG阳性早产儿出现胎膜早破、胎盘早剥、羊水过少、羊水污染和皮肤异常的几率明显高于阴性早产儿.结论 本次检测的新生儿HSV-1 IgG和HCMV IgG阳性率较高,且两者阳性的早产儿可能伴随胎膜早破等临床表现.
目的 瞭解新生兒單純皰疹病毒1型(herpes simplex virus type 1,HSV-1)和人巨細胞病毒( human cytomegalovirus,HCMV)血清抗體暘性情況及其與早產兒臨床錶現的關繫.方法 收集新生兒血清共178份(其中健康產婦順產新生兒臍帶血血清114份,早產兒外週血血清64份),分彆用HSV-I和HCMV IgG、IgM ELISA試劑盒進行檢測.採用x2檢驗對結果進行比較.結果 178名新生兒中,血清HSV-1 IgG暘性率為83.70％,IgM為陰性；HCMV IgG和IgM暘性率分彆為94.38％和0.56％.在114名順產新生兒中,HSV-1 IgG和IgM暘性率分彆為78.07％和0.00％,HCMVIgG和IgM暘性率分彆為94.74％和0.88％,HSV-1 IgG和HCMVIgG共暘性率為73.68％.在64名早產兒中,HSV-1 IgG和IgM暘性率分彆為93.75％和0.00％,HCMV IgG和IgM暘性率分彆為93.75％和0.00％,HSV-1 IgG和HCMV IgG共暘性率為87.50％.早產兒與順產兒的HCMV IgG暘性率差異無統計學意義(x2=0.07,P＞0.05)；但早產兒HSV-1 IgG暘性率以及HSV-1 IgG和HCMV IgG共暘性率均高于順產兒,且差異有統計學意義(x2值分彆為7.38和4.65,P值均＜0.05).HSV-1 IgG和HCMVIgG暘性早產兒齣現胎膜早破、胎盤早剝、羊水過少、羊水汙染和皮膚異常的幾率明顯高于陰性早產兒.結論 本次檢測的新生兒HSV-1 IgG和HCMV IgG暘性率較高,且兩者暘性的早產兒可能伴隨胎膜早破等臨床錶現.
목적 료해신생인단순포진병독1형(herpes simplex virus type 1,HSV-1)화인거세포병독( human cytomegalovirus,HCMV)혈청항체양성정황급기여조산인림상표현적관계.방법 수집신생인혈청공178빈(기중건강산부순산신생인제대혈혈청114빈,조산인외주혈혈청64빈),분별용HSV-I화HCMV IgG、IgM ELISA시제합진행검측.채용x2검험대결과진행비교.결과 178명신생인중,혈청HSV-1 IgG양성솔위83.70％,IgM위음성；HCMV IgG화IgM양성솔분별위94.38％화0.56％.재114명순산신생인중,HSV-1 IgG화IgM양성솔분별위78.07％화0.00％,HCMVIgG화IgM양성솔분별위94.74％화0.88％,HSV-1 IgG화HCMVIgG공양성솔위73.68％.재64명조산인중,HSV-1 IgG화IgM양성솔분별위93.75％화0.00％,HCMV IgG화IgM양성솔분별위93.75％화0.00％,HSV-1 IgG화HCMV IgG공양성솔위87.50％.조산인여순산인적HCMV IgG양성솔차이무통계학의의(x2=0.07,P＞0.05)；단조산인HSV-1 IgG양성솔이급HSV-1 IgG화HCMV IgG공양성솔균고우순산인,차차이유통계학의의(x2치분별위7.38화4.65,P치균＜0.05).HSV-1 IgG화HCMVIgG양성조산인출현태막조파、태반조박、양수과소、양수오염화피부이상적궤솔명현고우음성조산인.결론 본차검측적신생인HSV-1 IgG화HCMV IgG양성솔교고,차량자양성적조산인가능반수태막조파등림상표현.
Objective To understand serum positive rates of herpes simplex virus type 1 (HSV-1) and human cytomegalovirus (HCMV) in 178 neonats and their relationship with clinical manifestation of premature infants.Methods A total of 178 neonatal serum samples were collected,including 114 umbilical cord sera from normally delivered newborns and 64 blood sera from premature infants.IgG and IgM antibodies to HSV-1 and HCMV were detected with ELISA.The x2 test was adopted for the comparisons.Results In 178 neonats,HSV-1 IgG and IgM positive rates were 83.70％ and 0.00％,HCMV IgG and IgM positive were 94.38％ and 0.56％,respectively.In 114 normally delivered newborns,HSV-1 IgG and IgM positive rates were 78.07％ and 0.00％,HCMV IgG and IgM positive were 94.74％ and 0.88％,respectively.The positive rate of both HSV-1 IgG and HCMV IgG was 73.68％.In 64 premature infants,HSV-1 IgG and IgM positive rates were 93.75％ and 0.00％,HCMV IgG and IgM positive rates were 93.75％ and 0.00％,respectively.The positive rate of both HSV-1 IgG and HCMV IgG was 87.50％.The HCMV lgG positive rates between premature and normally delivered neonates were not statistically significant (x2 =0.07,P ＞ 0.05 ) ;while HSV-1 IgG positive rate and both HSV-1 IgG and HCMV IgG positive rate in premature neonates were significantly higher than those in normally delivered newborns (x2 =7.38,P ＜ 0.05 ; x2 =4.65,P ＜ 0.05 ).The premature infants with HSV-1 IgG and HCMV IgG positive had higher risk of premature rupture of membranes,placental abruption,oligohydramnios,amniotic fluid contamination and skin abnormalities.Conclusions The positive rates of HSV-1 IgG and HCMV IgG are high in neonates.The premature infants with HSV-1 IgG and HCMV IgG positive may be associated with premature rupture of membranes and other clinical manifestations.
