中华物理医学与康复杂志
中華物理醫學與康複雜誌
중화물리의학여강복잡지
CHINESE JOURNAL OF PHYSICAL MEDICINE AND REHABILITATION
2012年
4期
251-254
,共4页
早期环境%脑红蛋白%学习记忆%大鼠
早期環境%腦紅蛋白%學習記憶%大鼠
조기배경%뇌홍단백%학습기억%대서
Early environment%Neuroglobin%Learning%Memory
目的 观察早期不同环境养育对大鼠学习记忆能力及脑红蛋白(Ngb)的影响.方法 将45只新生雄性SD大鼠按随机数字表法分为丰富环境组、隔离环境组和正常对照组,每组大鼠15只.丰富环境组和隔离环境组于生后第1～28天分别接受早期丰富环境和隔离环境刺激,正常对照组常规饲养,3组大鼠均于生后第29天采用跳台试验评价大鼠学习记忆能力,并采用免疫组织化学方法测定大鼠Ngb表达情况.结果 跳台试验结果显示,丰富环境组大鼠学习成绩和记忆成绩分别为(9.67±0.49)分和(9.80±0.56)分,明显高于正常对照组的(8.67±0.72)分和(8.93±1.10)分,而隔离环境组学习记忆成绩最低分别为(7.07±1.98)分和(7.67±0.98)分,3组大鼠各项评分组间比较,差异均有统计学意义(P＜0.05)；丰富环境组大鼠触电潜伏期为(166.33±36.08)s,与正常对照组(108.93±73.26)s比较,差异有统计学意义(P＜0.05),而隔离环境组大鼠触电潜伏期为(44.93±45.03)s,与丰富环境组和正常对照组比较,差异均有统计学意义(P＜0.05).丰富环境组额叶皮质和海马Ngb的积分光密度(IOD)值分别为(224.56±89.09)和(127.70±28.16)；高于正常对照组的(141.33±45.10)和(92.69±28.52),差异有统计学意义(P＜0.05),而隔离环境组大鼠额叶皮质及海马Ngb的IOD值分别为(88.78±18.84)和(63.42±16.41),与丰富环境组和正常对照组比较,差异均有统计学意义(P＜0.05).结论 早期丰富环境刺激可提高大鼠的学习记忆能力,增加Ngb的表达；而隔离环境可减少Ngb的表达,使大鼠学习记忆能力受损.
目的 觀察早期不同環境養育對大鼠學習記憶能力及腦紅蛋白(Ngb)的影響.方法 將45隻新生雄性SD大鼠按隨機數字錶法分為豐富環境組、隔離環境組和正常對照組,每組大鼠15隻.豐富環境組和隔離環境組于生後第1～28天分彆接受早期豐富環境和隔離環境刺激,正常對照組常規飼養,3組大鼠均于生後第29天採用跳檯試驗評價大鼠學習記憶能力,併採用免疫組織化學方法測定大鼠Ngb錶達情況.結果 跳檯試驗結果顯示,豐富環境組大鼠學習成績和記憶成績分彆為(9.67±0.49)分和(9.80±0.56)分,明顯高于正常對照組的(8.67±0.72)分和(8.93±1.10)分,而隔離環境組學習記憶成績最低分彆為(7.07±1.98)分和(7.67±0.98)分,3組大鼠各項評分組間比較,差異均有統計學意義(P＜0.05)；豐富環境組大鼠觸電潛伏期為(166.33±36.08)s,與正常對照組(108.93±73.26)s比較,差異有統計學意義(P＜0.05),而隔離環境組大鼠觸電潛伏期為(44.93±45.03)s,與豐富環境組和正常對照組比較,差異均有統計學意義(P＜0.05).豐富環境組額葉皮質和海馬Ngb的積分光密度(IOD)值分彆為(224.56±89.09)和(127.70±28.16)；高于正常對照組的(141.33±45.10)和(92.69±28.52),差異有統計學意義(P＜0.05),而隔離環境組大鼠額葉皮質及海馬Ngb的IOD值分彆為(88.78±18.84)和(63.42±16.41),與豐富環境組和正常對照組比較,差異均有統計學意義(P＜0.05).結論 早期豐富環境刺激可提高大鼠的學習記憶能力,增加Ngb的錶達；而隔離環境可減少Ngb的錶達,使大鼠學習記憶能力受損.
목적 관찰조기불동배경양육대대서학습기억능력급뇌홍단백(Ngb)적영향.방법 장45지신생웅성SD대서안수궤수자표법분위봉부배경조、격리배경조화정상대조조,매조대서15지.봉부배경조화격리배경조우생후제1～28천분별접수조기봉부배경화격리배경자격,정상대조조상규사양,3조대서균우생후제29천채용도태시험평개대서학습기억능력,병채용면역조직화학방법측정대서Ngb표체정황.결과 도태시험결과현시,봉부배경조대서학습성적화기억성적분별위(9.67±0.49)분화(9.80±0.56)분,명현고우정상대조조적(8.67±0.72)분화(8.93±1.10)분,이격리배경조학습기억성적최저분별위(7.07±1.98)분화(7.67±0.98)분,3조대서각항평분조간비교,차이균유통계학의의(P＜0.05)；봉부배경조대서촉전잠복기위(166.33±36.08)s,여정상대조조(108.93±73.26)s비교,차이유통계학의의(P＜0.05),이격리배경조대서촉전잠복기위(44.93±45.03)s,여봉부배경조화정상대조조비교,차이균유통계학의의(P＜0.05).봉부배경조액협피질화해마Ngb적적분광밀도(IOD)치분별위(224.56±89.09)화(127.70±28.16)；고우정상대조조적(141.33±45.10)화(92.69±28.52),차이유통계학의의(P＜0.05),이격리배경조대서액협피질급해마Ngb적IOD치분별위(88.78±18.84)화(63.42±16.41),여봉부배경조화정상대조조비교,차이균유통계학의의(P＜0.05).결론 조기봉부배경자격가제고대서적학습기억능력,증가Ngb적표체；이격리배경가감소Ngb적표체,사대서학습기억능력수손.
Objective To explore the influence of environment early in life on learning and memory abilities and neuroglobin (Ngb) expression. Methods Forty-five newborn,male,Sprague-Dawley rats were randomly divided into an EE (enriched environment) group,an EI (isolated environment) group and a normal control group,with 15 rats in each group.The EE and EI group rats lived in those environments for their first 28 days of life.The step-down test was used to measure the rats'learning and memory abilities on the 29th day.Ngb expression was examined using immunohistochemical methods. Results The step-down test showed learning and memory scores for the EE group rats of (9.67±0.49) and (9.80±0.56) respectively,significantly higher than those of the control group,(8.67±0.72) and (8.93±1.10).The learning and memory abilities of the EI group were the lowest among the three groups,with scores of only (7.07±1.98) and (7.67±0.98 ),respectively.The latency of the first electric shock was (166.33±36.08)s in the EE group and significantly shorter than that in the control group (108.93±73.26) s.The EI group showed the longest average latency (44.93±45.03)s.Average Ngb expression as reflected by the integrated optical density in the EE group was (224.56±89.09) in the frontal cortex and (127.70 ± 28.16)in the hippocampus,a significant increase over expression in the control group.The Ngb expressions of the EI group were the lowest among the 3 groups-(88.78 ± 18.84) and ( 63.42 ± 16.41 ) in the frontal cortex and hippocampus respectively. Conclusions Early exposure to an enriched environment can increase Ngb expression and improve learning and memory ability,but early isolation will reduce Ngb expression and learning and memory function.
