中华消化内镜杂志
中華消化內鏡雜誌
중화소화내경잡지
CHINESE JOURNAL OF DIGESTIVE ENDOSCOPY
2008年
6期
309-312
,共4页
宁晓燕%房殿春%李宜成%郭丽萍%帖君%杨仕明%汪荣泉%彭贵勇%陈文生
寧曉燕%房殿春%李宜成%郭麗萍%帖君%楊仕明%汪榮泉%彭貴勇%陳文生
저효연%방전춘%리의성%곽려평%첩군%양사명%왕영천%팽귀용%진문생
端粒%RNA干扰%转录%基因表达
耑粒%RNA榦擾%轉錄%基因錶達
단립%RNA간우%전록%기인표체
Telomere%RNA interference%Transcription%Gene expression
目的 观察人端粒保护蛋白(hPOT1)RNA干扰对胃癌BGC823细胞端粒重复序列结合因子1(TRF1)、端粒重复序列结合因子2(TRF2)、端锚聚合酶(Tankyrase1)转录水平的影响.方法 采用半定量RT-PCR方法检测TRF1、TRF2和Tankyrase1在mRNA表达水平的改变.结果 hPOT1RNA干扰抑制细胞中hPOT1表达后,TRF1表达明显上调,TRF2和Tankyrase1明显下调.结论 人胃癌BGC823细胞中hPOT1表达下调伴随TRF1表达明显上调、TRF2和Tankyrase1表达明显下调,提示hPOT1和TRF1、TRP2、Tankyrase1三个端粒相关蛋白之间具有较为密切的联系,并且相互制约、相互影响.
目的 觀察人耑粒保護蛋白(hPOT1)RNA榦擾對胃癌BGC823細胞耑粒重複序列結閤因子1(TRF1)、耑粒重複序列結閤因子2(TRF2)、耑錨聚閤酶(Tankyrase1)轉錄水平的影響.方法 採用半定量RT-PCR方法檢測TRF1、TRF2和Tankyrase1在mRNA錶達水平的改變.結果 hPOT1RNA榦擾抑製細胞中hPOT1錶達後,TRF1錶達明顯上調,TRF2和Tankyrase1明顯下調.結論 人胃癌BGC823細胞中hPOT1錶達下調伴隨TRF1錶達明顯上調、TRF2和Tankyrase1錶達明顯下調,提示hPOT1和TRF1、TRP2、Tankyrase1三箇耑粒相關蛋白之間具有較為密切的聯繫,併且相互製約、相互影響.
목적 관찰인단립보호단백(hPOT1)RNA간우대위암BGC823세포단립중복서렬결합인자1(TRF1)、단립중복서렬결합인자2(TRF2)、단묘취합매(Tankyrase1)전록수평적영향.방법 채용반정량RT-PCR방법검측TRF1、TRF2화Tankyrase1재mRNA표체수평적개변.결과 hPOT1RNA간우억제세포중hPOT1표체후,TRF1표체명현상조,TRF2화Tankyrase1명현하조.결론 인위암BGC823세포중hPOT1표체하조반수TRF1표체명현상조、TRF2화Tankyrase1표체명현하조,제시hPOT1화TRF1、TRP2、Tankyrase1삼개단립상관단백지간구유교위밀절적련계,병차상호제약、상호영향.
Objective To investigate the effect of silence of human protection of telomeres 1 (hPOT1), which was induced by RNA interference, on expression of telomeric repeat factor 1 (TRF1), telomeric repeat factor 2 (TRF2) and Tankyrase 1 in human gastric cancer cell BGC823. Methods The ex-pression of TRF1 ,TRF2 and Tankyrasel at mRNA level were determined by semi-quantitative RT-PCR. Re-sults Significant increase in expression of TRFI, marked decrease of TRF2 and Tankyrase1 at mRNA level were observed in cells of hPOT1 siRNA. Conclusion The significant increase in expression of TRF1 and the marked decease in TRF2 and Tnakyrasel at mRNA level after the inhibited expression of hPOT1 in human gastric cancer cell BGC823 indicate that hPOTI is highly correlated with the expressions of other three te-lomere-specific binding proteins.
