临床肝胆病杂志
臨床肝膽病雜誌
림상간담병잡지
CHINESE JOURNAL OF CLINICAL HEPATOLOGY
2009年
3期
177-179
,共3页
耿书英%陈金军%万铁林%李咏茵%徐旭雯%侯金林
耿書英%陳金軍%萬鐵林%李詠茵%徐旭雯%侯金林
경서영%진금군%만철림%리영인%서욱문%후금림
肝炎病毒,乙型%非活动性HBsAg携带者%基因型%变异%核苷类似物
肝炎病毒,乙型%非活動性HBsAg攜帶者%基因型%變異%覈苷類似物
간염병독,을형%비활동성HBsAg휴대자%기인형%변이%핵감유사물
hepatitis B virus%lnactive HBsAg carrier%genotype%mutation%nucleot(s)ide analogue
目的 探讨核苷类似物(NA)治疗达到完全应答(CR)的HBeAg阳性CHB患者与非活动性HBsAg携带者(IAgCs)之间HBV病毒学特点的异同.方法 用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)以及部分PCR产物直接测序法对175例慢性HBV感染者进行HBV基因分型以及PC/BCP基因区变异检测,包括经NA治疗1年以上、达到CR的117例CHB患者(治疗组)和58例未行抗病毒治疗的IAgCs(对照组).结果 治疗组中1例A型、44例B型、16例C型,对照组中33例B型、10例C型、2例D型、1例基因型不明,两组的基因型构成比无统计学差异(P＞0.05);治疗组BCP区A1762T/G1764A检出率显著低于对照组(9.8% vs 41.4%,P＜0.01),PC区G1896A检出率亦明显低于对照组(14.0% vs 72.4%,P＜0.01).结论 NA治疗达到CR的HBeAg阳性CHB患者PC/BCP变异检出率低于IAgCs.
目的 探討覈苷類似物(NA)治療達到完全應答(CR)的HBeAg暘性CHB患者與非活動性HBsAg攜帶者(IAgCs)之間HBV病毒學特點的異同.方法 用聚閤酶鏈反應-限製性片段長度多態性(PCR-RFLP)以及部分PCR產物直接測序法對175例慢性HBV感染者進行HBV基因分型以及PC/BCP基因區變異檢測,包括經NA治療1年以上、達到CR的117例CHB患者(治療組)和58例未行抗病毒治療的IAgCs(對照組).結果 治療組中1例A型、44例B型、16例C型,對照組中33例B型、10例C型、2例D型、1例基因型不明,兩組的基因型構成比無統計學差異(P＞0.05);治療組BCP區A1762T/G1764A檢齣率顯著低于對照組(9.8% vs 41.4%,P＜0.01),PC區G1896A檢齣率亦明顯低于對照組(14.0% vs 72.4%,P＜0.01).結論 NA治療達到CR的HBeAg暘性CHB患者PC/BCP變異檢齣率低于IAgCs.
목적 탐토핵감유사물(NA)치료체도완전응답(CR)적HBeAg양성CHB환자여비활동성HBsAg휴대자(IAgCs)지간HBV병독학특점적이동.방법 용취합매련반응-한제성편단장도다태성(PCR-RFLP)이급부분PCR산물직접측서법대175례만성HBV감염자진행HBV기인분형이급PC/BCP기인구변이검측,포괄경NA치료1년이상、체도CR적117례CHB환자(치료조)화58례미행항병독치료적IAgCs(대조조).결과 치료조중1례A형、44례B형、16례C형,대조조중33례B형、10례C형、2례D형、1례기인형불명,량조적기인형구성비무통계학차이(P＞0.05);치료조BCP구A1762T/G1764A검출솔현저저우대조조(9.8% vs 41.4%,P＜0.01),PC구G1896A검출솔역명현저우대조조(14.0% vs 72.4%,P＜0.01).결론 NA치료체도CR적HBeAg양성CHB환자PC/BCP변이검출솔저우IAgCs.
Objective To investigate the virological differences between HBeAg positive hepatitis B patients with complete response to nucleot(s)ide anlogue treatment (NCRs) and inactive HBsAg carriers (IAgCs).Methods HBV genotypes, A1896 and T1762/A1764 mutations were determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) in combination with direct nucleotide sequencing in a cohort of 175 patients with chronic HBV infection, including 117 patients with chronic hepatitis B (CHB) who had achieved completed response under the treatment of nucleot(s)ide analog(case group), and 58 IAgCs who had achieved HBeAg seroconversion spontaneously (control group).Results No statistical difference was found in the constituent ratio of HBV genotypes between these two groups (1 genotype A, 44 genotype B, 16 genotype C in the case group and 33 genotype B, 10 genotype C, 2 genotype D, 1 uncertain genotype in the control group; P＞0.05). Prevalence of A1762T/G1764A and G18896A were significantly lower in the case group (9.8% and 14.0%) than that in the control group (41.4% and 72.4%; both P＜0.01).Conclusion Prevalences of PC/BCP mutations of NCRs are lower than that of IAgCs.
