蚌埠医学院学报
蚌埠醫學院學報
방부의학원학보
ACTA ACADEMIAE MEDICINAE BENGBU
2003年
1期
9-12
,共4页
黄谷良%林特夫%贾继辉%叶和平
黃穀良%林特伕%賈繼輝%葉和平
황곡량%림특부%가계휘%협화평
幽门螺杆菌%L型%胃肿瘤%胃炎
幽門螺桿菌%L型%胃腫瘤%胃炎
유문라간균%L형%위종류%위염
Helicobacter pylori%L-forms%stomach neoplasms%gastritis
目的:对幽门螺杆菌L型的生物学特性与致病性进行研究.方法:在体外用羧苄西林诱导幽门螺杆菌形成L型以研究其生物学特性.分别取幽门螺杆菌原菌与L型感染实验小鼠以测定其生物学特牲与致病性.取136份胃癌与30份慢性胃炎患者标本进行组织学检查和细菌分离.结果:体外诱导的L型与原菌在特性上差别很大,但仍能以免疫酶染色与PCR法识别.实验动物感染,在体内的L型呈圆球体,大多在胃与十二指肠黏膜引起间质性炎症,一只脾脏并见有恶性病变.临床胃癌标本检查见L型亦呈圆球体状,77.3%患者幽门螺杆菌抗原阳性.慢性胃炎胃液分离,除见有幽门螺杆菌外,并有多种细菌和L型存在.结论:幽门螺杆菌L型形态多变,其中以圆球体多见于病变部位,且导致间质性和肿瘤的形成.但与慢性胃炎的关系尚有待进一步研究.
目的:對幽門螺桿菌L型的生物學特性與緻病性進行研究.方法:在體外用羧芐西林誘導幽門螺桿菌形成L型以研究其生物學特性.分彆取幽門螺桿菌原菌與L型感染實驗小鼠以測定其生物學特牲與緻病性.取136份胃癌與30份慢性胃炎患者標本進行組織學檢查和細菌分離.結果:體外誘導的L型與原菌在特性上差彆很大,但仍能以免疫酶染色與PCR法識彆.實驗動物感染,在體內的L型呈圓毬體,大多在胃與十二指腸黏膜引起間質性炎癥,一隻脾髒併見有噁性病變.臨床胃癌標本檢查見L型亦呈圓毬體狀,77.3%患者幽門螺桿菌抗原暘性.慢性胃炎胃液分離,除見有幽門螺桿菌外,併有多種細菌和L型存在.結論:幽門螺桿菌L型形態多變,其中以圓毬體多見于病變部位,且導緻間質性和腫瘤的形成.但與慢性胃炎的關繫尚有待進一步研究.
목적:대유문라간균L형적생물학특성여치병성진행연구.방법:재체외용최변서림유도유문라간균형성L형이연구기생물학특성.분별취유문라간균원균여L형감염실험소서이측정기생물학특생여치병성.취136빈위암여30빈만성위염환자표본진행조직학검사화세균분리.결과:체외유도적L형여원균재특성상차별흔대,단잉능이면역매염색여PCR법식별.실험동물감염,재체내적L형정원구체,대다재위여십이지장점막인기간질성염증,일지비장병견유악성병변.림상위암표본검사견L형역정원구체상,77.3%환자유문라간균항원양성.만성위염위액분리,제견유유문라간균외,병유다충세균화L형존재.결론:유문라간균L형형태다변,기중이원구체다견우병변부위,차도치간질성화종류적형성.단여만성위염적관계상유대진일보연구.
Objective:The biological characteristics and pathogenicities of Helicobacter pylori L-forms were studied in this article.Methods:H.pylori were induced in vitro into L-forms with carbenicillin in media specially prepared for H.pylori L-forms and their biological characters were studied.The L-forms and their original vegetative forms were infected separately into different groups of laboratory mice to test for their pathogenicity.In clinical examinations,136 specimens taken from gastric cancer and 30 specimens of gastric juice from patients with chronic gastritis were subjected to histological examination and bacteriological isolation.Results:The L-forms induced in vitro varied greatly in characters from their vegetative forms,but could still be identified through immunoenzyme staining and PCR.In experimental infection,the L-forms induced in vivo were mostly in spherical form,that led to interstitial inflammations in mucosa of stomach and duodenum of all animals infected and with malignant change in spleen in 1 animal.In clinical examination of the gastric cancer H.pylori L-forms in spherical shape or positive in H.pylori antigen were found in 77.3% of the cases.In isolation of gastric juice from patients with chronic gastritis,L-forms of different bacteria species were found,besides that of H.pylori.Conclusions:H.pylori can be induced into L-forms in different shapes.The L-forms in spherical shape seems to be more pathogenic,that can lead to interstitial changes and cancer formation,but their relation to chronic gastritis still need further investigation.